Establishment and evaluation of a lung metastasis model of colorectal cancer in experimental animals
- VernacularTitle:Туршилтын амьтны уушгинд бүдүүн шулуун гэдэсний хорт хавдрын үсэрхийллийн загвар үүсгэн судалсан дүн
- Author:
Anujin D
1
;
Manaljav B
2
;
Barsbold M
2
;
Altanchimeg Ch
2
;
Otgonsuren B
2
;
Khuselt-Od T
3
;
Suvd-Erdene U
3
;
Enkhsaikhan L
4
,
5
;
Gansukh Ch
6
;
Juramt B
1
Author Information
1. Department of Anatomy, School of Bio-Medicine, MNUMS
2. Sci-Lab Student Club, MNUMS
3. Students of School of Bio-Medicine, MNUMS
4. Institute of Biomedical Sciences, MNUMS
5. Department of Immunology, School of Bio-Medicine, MNUMS
6. Department of Immunology, School of Bio-Medicine, MNUMS
- Publication Type:Other Types
- Keywords:
Experimental animal model;
Lung;
C57BL/6J mouse;
MC38
- From:
Mongolian Journal of Health Sciences
2025;88(4):19-23
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background:Currently, colorectal cancer (CRC) ranks as the third most common cancer and the second leading cause
of cancer-related mortality worldwide. CRC frequently metastasizes to the liver (50%), lungs (10–15%), peritoneum
(4%), bones (10.7%–23.7%), brain (0.3%–6%), and spinal cord. Approximately 35% of CRC cases are diagnosed before
distant metastasis, 36% upon lymph node involvement, and 23% after distant organ metastasis. Although several studies
have established primary tumor models in mice in our country, there are limited studies on experimental lung metastasis
models, prompting the need for this research.
Aim:To establish and evaluate a lung metastasis model of colorectal cancer in C57BL/6J mice using the MC38 cell line.
Materials and Methods:This study was conducted at the Institute of Biomedical Sciences, Mongolian National Uni
versity of Medical Sciences. Approval was obtained from the Ethics Review Board of the Mongolian National Univer
sity of Medical Sciences (2023/3-09) and all laboratory safety regulations and protocols were strictly followed. Male
C57BL/6J mice bred at the Experimental Animal Center of Mongolian National University of Medical Sciences were
used. MC38 murine colorectal carcinoma cells were cultured and injected intravenously (via the tail vein) at a concen
tration of 0.25×10⁶ cells per mouse (n=12) to induce lung metastasis. Histological analysis was subsequently performed.
Results:Histological examination revealed significant alterations in lung tissue architecture, characterized by areas of
dense infiltration by pleomorphic, hyperchromatic cells, disrupting the normal alveolar structure. No histological abnor
malities were observed in other organs.
Conclusion:Intravenous injection of MC38 colorectal adenocarcinoma cells into the tail vein of C57BL/6J mice success
fully induced lung metastases, characterized by hyperchromatic, pleomorphic cell infiltrates forming glandular structures
within the lung parenchyma.
- Full text:202505201723022569619-23.pdf
