HPLC method for quantification of salidroside for quality control of golden root (Rhodiola Rosea L, Rhodiola Crenulata L.) dry extract
- VernacularTitle:Ягаан мүгэз (Rhodiola Rosea L. Rhodiola Crenulata L.) ургамлын хуурай ханданд салидрозидийн тооны тодорхойлолт хийх өндөр идэвхт шингэний хроматографийн арга
- Author:
Battulga B
1
;
Badamtsetseg S
1
;
Lkhaasuren R
1
;
Odontuya G
2
;
Khurelbaatar L
1
Author Information
1. Drug Research Institute
2. Mongolian University of Pharmaceutical Science
- Publication Type:Journal Article
- Keywords:
Salidroside;
High-performance liquid chromatography;
quality control;
method validation
- From:
Mongolian Pharmacy and Pharmacology
2024;25(2):52-57
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background: The high-performance liquid chromatography (HPLC) method was developed to select
salidroside in tablet formulation dietary supplements, raw material containing other components. Further,
the proposed method was validated for linearity, precision (system precision, method precision, intermediate
or inter-day precision), and accuracy, stability in analytical solution, syst em suitability, and ruggedness.
The developed method exhibited the best results in terms of the validation above parameters. The other
components and additives did not interfere with their determinations. The method was found to be selective,
simple, economical, accurate, reproducible, rapid, and reliable for routine estimation purposes of salidroside
in golden root dry extract. The goal of this study was to develop the validation method of salidroside in the
dietary supplement.
Material and Methods: The Rhodiola rosae L. dry extract was supplied Arshin Co.ltd in People’s Republic of
China. The standard salidroside was supplied from Sigma Aldrich Co Ltd. We used solvents for HPLC grade
(methanol, acetonitrile). Chromatographic conditions: A gradient HPLC (Shimadzu CBM20AD) with serial
dual plunger pump; analytical column: Shimadzu GIST С18 150 x 4.6 mm, particle size 5 μm; flow rate: 1
ml/min; column temperature: 400C, detection: UV 275 nm. Chromatographic procedure: 20 μl of the mixed
standard preparation and assay (sample) preparation were separately injected into the chromatography, the
chromatograms were recorded, and the responses for the major peaks were measured. The run time was
approximately 15 minutes.
Results: The calibration curves for the salidroside were made by plotting the peak area versus the
concentration for each analyte using regression analysis. Each calibration curve was obtained using six levels
of concentrations in the range of 100-800 µg/mL. The linear correlation coefficient (r2=1) for all calibration
curves was higher than 1 for all analytes. The LOD and LOQ for salidroside were golden root dry extract
in 8.788 µg/mL and 26.61 µg/mL, respectively. Accuracy and precision were assessed by analyzing five
samples independently prepared at low, middle, and high concentrations. The RSD values of repeatability
and intermediate precision were below 1.12%, 1.19 and 1.79%. The accuracy remains between 91 to 109%.
The resulting accuracy data were satisfactory for the quantitative analysis of salidroside in golden root dry
extract. This article presents a simple, accurate, reproducible, and thoroughly validated HPLC-based method
for qualitative and quantitative analysis of salidroside, as part of the quality assessment of golden root dry
extract.
- Full text:2025051315234104051MPPJ-2024-25(2)-52-57.pdf
