Study on the safety and pharmacological effect on improving dyspepsia of Shuangshu decoction in rats
- VernacularTitle:双术汤对大鼠的安全性及改善消化性不良的药效作用研究
- Author:
Xinyuan CHEN
1
;
Changzhou XIONG
1
;
Jiongfen LI
1
;
Kangyi YU
2
;
Huan XU
2
;
Yingxia WANG
2
;
Dan LIAO
3
;
Junyu TAO
1
,
4
,
5
;
Ziyi YANG
6
;
Caizhi LIN
1
Author Information
1. School of Graduate，Guangxi University of Chinese Medicine，Nanning 530200，China
2. Faculty of Chinese Medicine Science，Guangxi University of Chinese Medicine，Nanning 530200，China
3. School of Basic Medicine，Guangxi University of Chinese Medicine，Nanning 530200，China
4. Guangxi Key Laboratory of Translational Medicine for Treating High-incidence Infectious Diseases with Integrative Medicine，Nanning 530200，China
5. Key Laboratory of Characteristic Experimental Animal Models of Guangxi，Nanning 530200，China
6. School of Acupuncture-moxibustion and Tuina，Chengdu University of Traditional Chinese Medicine，Chengdu 610075，China
- Publication Type:Journal Article
- Keywords:
Shuangshu decoction;
safety;
pharmacodynamics;
acute toxicity;
subacute toxicity;
functional dyspepsia
- From:
China Pharmacy
2025;36(9):1059-1064
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the safety of Shuangshu decoction in rats and its efficacy in improving functional dyspepsia （FD） in rats. METHODS In safety test， 40 rats were divided into blank control group， Shuangshu decoction low-dose， medium- dose and high-dose groups [108， 216， 324 g/（kg·d）， calculated by raw medicine， the same applies below]； they were given relevant medicine intragastrically， for continuous 14 days. The mortality and toxic reactions of rats were recorded， and the organ indexes of the liver， kidney， spleen， lung and heart of rats were calculated； the pathological morphological changes in the liver， kidney， spleen， lung， heart， stomach， duodenum， and colon were observed to evaluate the acute toxicity of Shuangshu decoction. Another 40 rats were grouped and administered in the same way for 30 consecutive days. The mortality and toxic reactions of the rats were recorded， and the corresponding organ indexes were calculated. The pathological morphological changes in the corresponding organs were observed， and blood routine and serum biochemical indicators were measured， in order to assess the subacute toxicity of Shuangshu decoction. In pharmacodynamic experiments: 50 rats were divided into blank control group， model group， and Shuangshu decoction low-， medium-， and high-dose groups （9.45， 18.9， 37.8 g/kg）， with 10 rats in each group. Except for blank control group， rats in all other groups were used to establish the FD rat model by subcutaneous injection of loperamide （3.5 mg/kg）. Rats in each group were administered the corresponding drug solution/normal saline intragastrically， once a day， for 14 consecutive days. After the last medication， fecal moisture content， intestinal propulsion rate， gastric emptying rate and serum level of motilin were all detected， and interstitial cell of Cajal （ICC） ultrastructure of rats was observed in colon tissue. RESULTS The safety experiments showed that no death occurred in each dose group， and no significant difference was found in organ coefficient， routine blood and serum biological index， compared to blank control group （P＞0.05）； no abnormality was found in organ appearance and pathological sections. The results of the pharmacodynamic experiments showed that， compared with the blank control group， the fecal moisture content， gastric emptying rate， intestinal propulsion rate， and serum motilin levels in the model group were significantly decreased （P＜0.05）； in the colonic tissue， the mitochondria in the ICC exhibited severe swelling with the disappearance of cristae， and the endoplasmic reticulum was dilated. Compared with model group， the rats in Shuangshu decoction high-dose group showed significant increases in the above quantitative indicators （P＜ 0.05）； additionally， there was a large number of mitochondria in the ICC of the colonic tissue， with clear cristae and regular arrangement. CONCLUSIONS Shuangshu decoction is safe and has a beneficial improving effect on FD rats； its mechanism of action may be related to the regulation of gastrointestinal hormone expression to promote gastric emptying and intestinal propulsion， as well as the repair of mitochondrial structure in ICCs to restore gastrointestinal function.
