Quality evaluation of Mongolian medicine Sendeng-4 based on qualitative and quantitative analysis combined with chemical pattern recognition
- VernacularTitle:基于定性、定量及化学模式识别分析的蒙药森登-4质量评价
- Author:
Fengye ZHOU
1
;
Jun LI
2
,
3
;
Qian ZHANG
2
,
3
;
Rongjie LI
1
;
Wei ZHANG
2
,
3
;
Jing LIU
2
,
3
;
Fang WANG
2
,
3
;
Shengnan LI
4
Author Information
1. Dept. of Pharmacy，the Affiliated Hospital of Inner Mongolia Medical University，Hohhot 010030，China
2. College of Pharmacy，Inner Mongolia Medical University，Hohhot 010110，China
3. Inner Mongolia Autonomous Region Engineering Research Center of New Pharmaceutical Screening，Hohhot 010110，China
4. Dept. of Endocrinology，the Affiliated Hospital of Inner Mongolia Medical University，Hohhot 010030，China
- Publication Type:Journal Article
- Keywords:
Sendeng-4;
qualitative analysis;
quantitative analysis;
chemical pattern recognition analysis;
content
- From:
China Pharmacy
2025;36(9):1040-1045
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To evaluate the quality of Mongolian medicine Sendeng-4 based on qualitative and quantitative analysis combined with chemical pattern recognition， in order to provide the reference for its quality control. METHODS The chemical components in Sendeng-4 were analyzed qualitatively by HPLC-Q-Exactive-MS. The contents of 16 components （methyl gallate， ethyl gallate， epicatechin， dihydromyricetin， genipin-1-O-β-D-gentiobioside， caffeic acid， catechin， corilagin， deacetylasperulosidic acid methyl ester， rutin， geniposide， luteolin， myricetin， quercetin， ferulic acid， and toosendanin） in 15 batches of Sendeng-4 （sample S1-S15） were quantitatively analyzed by HPLC-MS/MS. Cluster analysis （CA）， principal component analysis （PCA）， and orthogonal partial least squares discriminant analysis were conducted and variable importance projection （VIP） value greater than 1 was used as the index to screen the differential components. RESULTS A total of 73 chemical components were identified in Sendeng-4， including 20 flavonoids， 16 tannins， 14 organic acids， etc. According to the quantitative analysis， the results exhibited that the average contentsthe of above 16 components in 15 batches of Sendeng-4 were 3.683-7.730， 2.391-6.952， 2 275.538-4 377.491， 2 699.188-3 537.924， 858.266-1 377.393， 3.366-11.003， 140.624-315.683，414.629-978.334， 285.501-1 510.457， 27.799-48.325， 3 625.415-6 309.563， 0.506-0.656， 442.337-649.283， 47.093-59.736， 12.942-15.822， 127.738-326.649 μg/g， respectively. According to the results of CA and PCA， 15 batches of samples could be clustered into two categories: S1-S3， S5-S6， S9-S10 and S13 were clustered into one category； S4， S7-S8， S11-S12， S14-S15 were clustered into one category. VIP values of geniposide， epicatechin， deacetylasperulosidic acid methyl ester and genipin-1-O- β-D-gentiobioside were all greater than 1. CONCLUSIONS HPLC-Q-Exactive-MS and HPLC-MS/MS techniques are employed for the qualitative and quantitative analysis of Sendeng-4. Through chemical pattern recognition analysis， four differential components are identified: geniposide， epicatechin， deacetylasperulosidic acid methyl ester， and genipin-1-O-β-D-gentiobioside.
