Characteristic ion Identification of Different Original Haliotidis Concha and Its Counterfeits
10.13422/j.cnki.syfjx.20250968
- VernacularTitle:不同基原石决明及其伪品的特征离子鉴别
- Author:
Xiaojie LIANG
1
;
Guowei LI
2
;
Lin ZHOU
2
;
Qiping HU
2
;
Muxiang LUO
3
;
Jiehao TANG
4
;
Xiangdong CHEN
2
;
Liye PAN
2
;
Dongmei SUN
2
Author Information
1. Guangzhou University of Chinese Medicine，Guangzhou 510006，China
2. Guangdong Provincial Enterprise Key Laboratory of Traditional Chinese Medicine Formula Granule，Guangdong Efong Pharmaceutical Co. Ltd.，Foshan 528244，China
3. Southern Medical University，Foshan 528244，China
4. Guangdong Pharmaceutical University，Guangzhou 510006，China
- Publication Type:Journal Article
- Keywords:
Haliotidis Concha;
characteristic ions;
ultra-high performance liquid chromatography-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry（UPLC-Q-Exactive-Orbitrap-MS/MS）;
UPLC-triple quadrupole tandem mass spectrometry（UPLC-QqQ-MS/MS）;
identification;
quality evaluation
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2025;31(11):263-269
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo establish a method for the identification of Haliotidis Concha and its counterfeits， and to improve its quality evaluation method. MethodsA total of 17 batches of Haliotis discus hannai， 4 batches of H. ruber， 3 batches of H. laevigata， 3 batches of H. ovina， 3 batches of H. diversicolor， 3 batches of H. asinina， 3 batches of H. iris were collected. Ultra-high performance liquid chromatography-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry（UPLC-Q-Exactive-Orbitrap-MS/MS） was used to analyze the hydrolysates of different original Haliotidis Concha and its counterfeits， and the potential characteristic ions of each species were screened by Venn diagram. UPLC-triple quadrupole tandem mass spectrometry（UPLC-QqQ-MS/MS） was used to validate the characteristic ions， and the specific detection method of the characteristic ions was established. ResultsA total of 1 182， 167， 47， 89， 104， 203， 424 potential characteristic ions were screened from H. discus hannai， H. ruber， H. laevigata， H. ovina， H. diversicolor， H. asinina and H. iris， respectively. And 9 characteristic ions were selected. The precision， stability and repeatability of the 9 characteristic ions in the established identification method met the requirements. Different original Haliotidis Concha and its counterfeits could detect their own characteristic ions， including m/z 631.83-886.48（double charge） and m/z 631.83-443.74（double charge） of H. discus hannai， m/z 699.28-232.11（double charge） and m/z 699.28-544.27（double charge） of H. ruber， m/z 535.76-752.37（double charge） and m/z 535.76-548.28（double charge） of H. laevigata， m/z 708.35-442.28（double charge） and m/z 708.35-215.14（double charge） of H. ovina， m/z 561.33-614.86（triple charge）， m/z 561.33-468.28（triple charge）， m/z 608.29-618.32（double charge） and m/z 608.29-390.21（double charge） of H. diversicolor， m/z 769.85-274.10（double charge）， m/z 769.85-532.75（double charge）， m/z 827.43-646.36（single charge）， m/z 827.43-257.12（single charge） of H. asinina， and m/z 468.24-576.29（double charge） and m/z 468.24-505.26（double charge） of H. iris. ConclusionIn this study， a total of 9 characteristic ions are screened from 6 kinds of original Haliotidis Concha and its counterfeits， and a specific identification method is established， which is helpful to solve the limitations of the existing quality evaluation methods of Haliotidis Concha， and provide a basis for the production， circulation and medication quality.
