Gypenoside L Regulates piR-hsa-2804461/FKBP8/Bcl-2 Axis to Promote Apoptosis and Inhibit Ovarian Cancer
10.13422/j.cnki.syfjx.20250413
- VernacularTitle:绞股蓝皂苷L调控piR-hsa-2804461/FKBP8/Bcl-2轴促进细胞凋亡抗卵巢癌机制
- Author:
Yuanguang DONG
1
;
Yinying SUN
1
;
Mingdian YUAN
2
;
Ying YANG
1
;
Jiaxin WANG
1
;
Jingxuan ZHU
1
;
Nan SONG
1
Author Information
1. Liaoning University of Traditional Chinese Medicine（TCM）， Shenyang 110847，China
2. Liaoning Provincial Hospital of TCM， Shenyang 110033，China
- Publication Type:Journal Article
- Keywords:
ovarian cancer;
gypenoside L;
piR-hsa-2804461;
FKBP8;
B-cell lymphoma-2 （Bcl-2）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2025;31(11):98-106
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the molecular mechanism by which gypenoside L （Gyp-L） promotes apoptosis and inhibits ovarian cancer （OC） through the FK506-binding protein （FKBP） prolyl isomerase 8 （FKBP8）/B-cell lymphoma-2 （Bcl-2） axis， with the piR-hsa-2804461 pathway as a breakthrough point. MethodsThe effects of different concentrations of Gyp-L and cis-platinum on the proliferation of OVCAR3 cells were determined by the cell count kit-8 method to identify the appropriate intervention concentration for subsequent experiments. OVCAR3 cells were allocated into blank， low-dose Gyp-L （Gyp-L-L， 50 µmol·L-1）， high-dose Gyp-L （Gyp-L-H， 100 µmol·L-1）， and cis-platinum （15 µmol·L-1） groups. The migration， colony formation， and apoptosis of OVCAR3 cells were detected by the cell scratch assay， colony formation assay， and flow cytometry， respectively. The mRNA levels of piR-hsa-2804461 and FKBP8/Bcl-2 axis-related genes in OVCAR3 cells were determined by Real-time PCR， and the expression levels of FKBP8/Bcl-2 axis-related proteins were determined by simple Western blot. Further， an OVCAR3 cell model with piR-hsa-2804461 knocked out was constructed. The cells were allocated into blank， NC-inhibitor， inhibitor， NC-inhibitor+Gyp-L， and inhibitor+Gyp-L groups. The colony formation of OVCAR3 cells was detected by the colony formation assay. The mRNA levels of piR-hsa-2804461 and FKBP8/Bcl-2 axis-related genes and the expression levels of FKBP8/Bcl-2 axis-related proteins were determined by Real-time PCR and simple Western blotting， respectively. ResultsGyp-L inhibited the migration and proliferation （P<0.01）， promoted the apoptosis （P<0.05）， up-regulated the mRNA level of piR-hsa-2804461 （P<0.05）， and down-regulated the mRNA and protein levels of FKBP8 and Bcl-2 （P<0.05） in OVCAR3 cells. Furthermore， Gyp-L increased the mRNA and protein levels of Bcl-2-associated X protein （Bax）， cysteinyl aspartate-specific proteinase （Caspase）-3， and Caspase-9， which are related to the FKBP8/Bcl-2 axis （P<0.05）. ConclusionGyp-L may promote apoptosis by regulating the piR-hsa-2804461/FKBP8/Bcl-2 axis， thus affecting the occurrence of ovarian cancer.
