Etiological characteristics and molecular evolution of the first mpox case in Huai’an City of Jiangsu Province
10.16250/j.32.1915.2024260
- VernacularTitle:江苏省淮安市首例猴痘病例病原学特征与分子进化研究
- Author:
Pengfei YANG
1
,
2
;
Fang HE
1
,
2
;
Qingli YAN
1
,
2
;
Heyuan GENG
3
;
Tong GAO
1
,
2
;
Qiang GAO
1
,
2
;
Chenglong XIONG
4
;
Haiyan PENG
5
Author Information
1. Huai’an Municipal Center for Disease Control and Prevention, Huai’an, Jiangsu 223001, China
2. Huai’an Municipal Key Laboratory of Emergency Detection for Public Health Incidents, Huai’an, Jiangsu 223001, China
3. Jiangsu International Travel Health Care Center (Nanjing Customs Port Clinic), China
4. School of Public Health, Fudan University, China
5. Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, Jiangsu 210009, China
- Publication Type:Journal Article
- Keywords:
Mpox;
Mpox virus;
Etiological characteristic;
Molecular evolution;
Molecular transmission network;
Huai’an City
- From:
Chinese Journal of Schistosomiasis Control
2025;37(1):85-92
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the virus subtypes, molecular evolutional and molecular transmission network features of the first confirmed mpox case in Huai’an City, Jiangsu Province, so as to provide insights into understanding of the transmission and evolution dynamics of mpox virus and formulation of the mpox control strategy in the city. Methods Genomic DNA was extracted from swabs of the first confirmed mpox case’s skin lesions in Huai’an City, and the amplicon sequencing library was constructed using the hypersensitive mpox virus whole-genome capture kit. High-throughput sequencing was performed using the GridION X5 nanopore sequencer on the Nanopore sequencing platform, and single nucleotide polymorphism (SNP) analysis of mpox virus genome sequences was performed following sequence assembly. In addition, phylogenetic analysis, genetic genealogy and molecular traceability analysis were performed. Results The virus whole genome sequence of the first confirmed mpox case was successfully obtained by high-throughput sequencing, with a full length of 197 182 bp, and was named hMpxV/China/JS-HA01/2023, which belonged to the clade IIb (West African clade) lineage B.1.3. Compared with the mpox virus reference sequence MPXV-M5312_HM12_Rivers-001 (GenBank accession number: NC_063383), the genome sequence of the Huai’an virus isolate carried 86 SNPs, including 40 SNPs in the coding region as non-synonymous mutations and 73 SNPs as nucleotide mutations caused by APOBEC3 (APOBEC3). Of the 97 mpox virus gene sequences, 79 sequences were included in the molecular network (81.44%), and the threshold of the genetic distance accessed to the network was 0.35/105. There were two large molecular transmission clusters and one scattered cluster in the molecular transmission network of the mpox virus, andthehMpxV/China/JS-HA01/2023 sequence was located in the large cluster. The 97 gene sequences formed 92 haplotypes, including three shared haplotypes Hap_4, Hap_6 and Hap_38, and an exclusive haplotype Hap_1 of hMpxV/China/JS-HA01/2023 generated from mutation of the exclusive haplotype Hap_43, while the exclusive haplotype Hap_43 was generated from mutation of the shared haplotype Hap_38. Conclusions The whole genome sequence of the mpox virus isolated from the first confirmed mpox case in Huai’an City has been successfully obtained, and the molecular evolutionary and molecular transmission network characteristics of the virus have been preliminarily understood.
