Rapid determination of tramadol in urine by surface-enhanced Raman spectroscopy

VernacularTitle:表面增强拉曼光谱法快速测定尿液中曲马多的研究
Author: Xiaojing YAO ¹ ; Peiying JI ¹ ; Feng LU ² ; Guorong SHI ³ ; Xiang FU ³
Author Information

1. Shanghai Yangpu District Kongjiang Hospital, Shanghai 200082, China.
2. Department of Pharmaceutical Analysis, School of Pharmacy, Naval Medical University, Shanghai 200433, China.
3. Shanghai Yangpu District Shidong Hospital, Shanghai 200438, China.
Publication Type:Originalarticles
Keywords: tramadol; urine; surface-enhanced Raman spectroscopy; potential inappropriate medication
From: Journal of Pharmaceutical Practice and Service 2025;43(4):185-189
CountryChina
Language:Chinese
Abstract: Objective To establish a method for rapid detection of tramadol in urine by liquid-liquid extraction（LLE）-surface-enhanced Raman spectroscopy （SERS）. Methods Tramadol was extracted from urine with chloroform∶isopropyl alcohol （9∶1） extractant and detected in urine samples by enhanced Raman spectroscopy （wavelength 785 nm）. Results The quantitative curve of tramadol was Y=204.35 X−465.62, r=0.9952, and the linear range was 1-100 μg/ml. The detection limit of tramadol by this method （S/N=3） was 0.53 μg/ml. The sensitivity of SERS was higher than that of conventional methods, and it had reasonable reproducibility. Conclusion This method is simple, efficient and economical, and can be used for qualitative and quantitative analysis of tramadol personalized medicine.