Operation guide for the visual electrophysiology data collection in small animals(2025)
10.3980/j.issn.1672-5123.2025.5.01
- VernacularTitle:小动物视觉电生理数据采集操作规范指南(2025)
- Author:
Yi SHAO
1
;
Shiying LI
1
;
Biao YAN
1
;
Biao YAN
1
;
Biao YAN
1
;
Biao YAN
1
;
Biao YAN
1
;
Biao YAN
1
Author Information
1. Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China
- Publication Type:Journal Article
- Keywords:
small animal visual electrophysiology;
visual electrophysiology;
neuroscience;
electroretinogram;
visual evoked potential operation guide
- From:
International Eye Science
2025;25(5):689-697
- CountryChina
- Language:Chinese
-
Abstract:
Visual electrophysiology measurements have become a routine method of functional examination in ophthalmology. Small animal visual electrophysiology is an important tool for exploring the functionality of the visual system in small animals, finding widespread applications in neuroscience and drug research and development. This guide aims to offer a standardized operational guide for the operation of visual electrophysiological norms in small animals to ensure the accuracy and repeatability of the test. The study emphasizes different types of visual electrophysiological tests, such as electroretinogram(ERG)and visual evoked potential(VEP), evoked in small animals, and their application in different disease models. Detailed descriptions are provided regarding the selection and preparation of experimental animals, including the requirements of animal species, anesthesia methods and test environment. In terms of operational procedures, this guide highlights the correct electrode placement, the selection of stimulus parameters, and the key steps for signal acquisition and processing. According to different animal models, the corresponding operation suggestions were provided, and the troubleshooting methods of common problems were introduced. Beyond fundamental operations, this guide also focuses on the interpretation and reporting of test results. It explains various types of electrophysiological waveforms. In summary, this operational specification for small animal visual electrophysiology provides a comprehensive and detailed framework for researchers to ensure the standardization and reliability of tests. By following these guidelines, researchers can effectively utilize small animal visual electrophysiology techniques to gain insight into the function and abnormalities of the visual system.
