Exploring Mechanism of Breast Cancer Prevention by Liuwei Dihuangwan， Erzhiwan and Zuoguiwan Based on Macrophage Phagocytosis
10.13422/j.cnki.syfjx.20250961
- VernacularTitle:基于巨噬细胞吞噬功能探讨六味地黄丸、二至丸、左归丸预防乳腺癌发生的机制
- Author:
Lixiang ZHENG
1
;
Yishan CHEN
1
;
Wei QUAN
1
;
Youbao ZHONG
1
;
Zifeng GUO
1
Author Information
1. Jiangxi University of Chinese Medicine，Nanchang 330004，China
- Publication Type:Journal Article
- Keywords:
breast cancer;
prevention;
Zuoguiwan;
Liuwei Dihuangwan;
Erzhiwan;
macrophage phagocytosis;
protein expression
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2025;31(10):10-17
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of three traditional Chinese medicine formulas for nourishing kidney Yin on the occurrence of breast cancer， including Zuoguiwan（ZGW）， Liuwei Dihuangwan（LWDHW） and Erzhiwan（EZW）， and to explore their preventive mechanisms from the phagocytic function of macrophages. MethodsTen-month-old post-breeding female mice were randomly divided into the blank group， soy isoflavone group（0.13 g·kg-1·d-1， mixed with feed）， ZGW group（2.34 g·kg-1·d-1）， LWDHW group（0.56 g·kg-1·d-1）， and EZW group（4.68 g·kg-1·d-1）. The mice were palpated every 3 d until the age of 18 months， and those with detected lumps were confirmed for tumor development through hematoxylin-eosin（HE） staining， and the incidence of breast cancer in mice was calculated. A total of 40 SPF-grade female SD rats were randomly divided into the blank serum group（physiological saline）， ZGW drug-containing serum group（16.20 g·kg-1·d-1of ZGW）， LWDHW drug-containing serum group（3.89 g·kg-1·d-1 of LWDHW） and EZW drug-containing serum group（32.40 g·kg-1·d-1 of EZW）， each group was orally administered 3 times a day for 5 consecutive days. On the 5th day， blood was collected from the abdominal aorta 1 h after the last gavage to prepare drug-containing serum. The effect of drug-containing serum with different concentrations on the viability of RAW264.7 cells was assessed by cell counting kit-8（CCK-8）. Taking 20% of drug-containing serum concentration as the research object， its effect on the expression levels of major histocompatibility complex-Ⅱ（MHC-Ⅱ）， CD80 and CD86 on the surface of RAW264.7 cells was detected by immunofluorescence（IF）， the phagocytic function of RAW264.7 cells was examined by flow cytometry， and the levels of interleukin-6（IL-6） and nitric oxide（NO） in RAW264.7 cells in the conditioned medium（CM） co culture system of 4T1 cells were detected by enzyme-linked immunosorbent assay（ELISA） and Griess assay. ResultsAfter prophylactic administration， the tumor incidence rates in the soy isoflavone group（4%）， ZGW group（4%）， LWDHW group（4%）， and EZW group（6%） were lower than that in the blank group（8%）. Compared with the blank serum group， the drug-containing serum of the three nourishing kidney Yin formulas could enhance the expression levels of MHC-Ⅱ， CD80 and CD86 on the surface of RAW264.7 cells， enhance phagocytic ability towards tumor cells， and reduce the content of IL-6 and increase the level of NO（P<0.05， P<0.01）. ConclusionThe three nourishing kidney Yin formulas can reduce the incidence of tumor in mice， the mechanism may be related to activating RAW264.7 cells， increasing their phagocytosis to breast cancer cells， and regulating the secretion of IL-6 and NO.
