Effects of Tripterygium wilfordii multiglycoside on renal injury in rats with diabetic nephropathy
- VernacularTitle:雷公藤多苷对糖尿病肾病大鼠肾损伤的影响
- Author:
Chong ZHANG
1
,
2
;
Chundong SONG
1
,
2
;
Mo WANG
3
;
Shuang LIANG
1
,
2
;
Xiaoxiao GUO
1
,
2
;
Hanhan ZHANG
1
,
2
;
Peijia LI
1
,
2
;
Ke SONG
1
,
2
;
Chenchen CHEN
1
Author Information
1. Second Ward of Nephrotic Purpura，Paediatric Hospital of the First Affiliated Hospital of Henan University of Chinese Medicine，Zhengzhou 450099，China
2. College of Pediatrics，Henan University of Chinese Medicine，Zhengzhou 450046，China
3. Dept. of Nephrology and Immunology，Children’s Hospital of Chongqing Medical University，400042，China
- Publication Type:Journal Article
- Keywords:
Tripterygium wilfordii multiglycoside;
diabetic nephropathy;
renal injury;
autophagy;
p53/miR-214/ULK1 axis
- From:
China Pharmacy
2025;36(7):815-819
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effects of Tripterygium wilfordii multiglycoside （TWM） on renal injury in diabetic nephropathy （DN） rats through tumor protein p53/microRNA-214 （miR-214）/UNC-51-like kinase 1 （ULK1） axis. METHODS Male SD rats were randomly divided into normal group （n＝6） and modeling group （n＝28）； the modeling group was fed with high fat and high glucose plus intraperitoneal injection of streptozotocin to establish DN model. The modeled rats were randomly divided into model group， valsartan group [8.33 mg/（kg·d）] and TWM group[6.25 mg/（kg·d）]， with 8 rats in each group. Rats in each group were gavaged with the corresponding medication or normal saline， once a day， for 6 consecutive weeks. After the last medication， liver and renal function indexes [24 h urinary total protein （24 h-UTP）， blood urea nitrogen （BUN）， serum creatinine （SCr）， albumin （ALB）， alanine transaminase （ALT）]， blood lipid indexes （triglycerides， total cholesterol） and blood glucose index （fasting blood glucose） in urine/blood sample of rats were detected in each group. Renal pathologic change was observed， protein and mRNA expressions of p53， ULK1， Beclin-1 and microtubule-associated protein 1 light chain 3 （LC3）， and expression of miR-214 in renal tissue were also determined. RESULTS Compared with the normal group， the renal tubular epithelium of rats in the model group showed obvious edema， cell swelling， accompanied by lymphocyte infiltration； the levels of 24h-UTP， BUN， SCr， ALT and glycolipid indexes， the expressions of p53 protein and mRNA， as well as the expression of miR-214 in rats in the model group and administration groups were significantly increased or up-regulated， while ALB level， LC3-Ⅱ/LC3-Ⅰ， the expressions of LC3 mRNA， the expressions of ULK1， Beclin-1 protein and mRNA were significantly decreased or down-regulated （P＜0.01）. Compared with the model group， the histopathological damage of the kidney in rats was improved in administration groups； the levels of 24 h-UTP， BUN， SCr， ALT and glycolipid indexes， the expressions of p53 protein and mRNA， as well as the expression of miR-214 were all significantly decreased or down-regulated， while ALB level， LC3-Ⅱ/LC3-Ⅰ， the expressions of LC3 mRNA， the expressions of ULK1 and Beclin-1 protein and mRNA were significantly increased or up-regulated （P＜0.01）. CONCLUSIONS TG can alleviate renal damage in DN rats， and improve their liver and renal function， as well as glucose and lipid levels. These effects may be related to the regulation of the p53/miR-214/ULK1 axis and the restoration of cellular autophagy.
