Effects of long non-coding RNA taurine up-regulated gene 1 on the proliferation, migration and angiogenesis of gastric cancer cells
- VernacularTitle:长链非编码RNA牛磺酸上调基因1对胃癌细胞增殖、迁移和血管生成的影响
- Author:
Peng FAN
1
;
Yujie HUANG
;
Xiangyu XIE
;
Liuhua WANG
;
Daorong WANG
Author Information
- Publication Type:Research Article
- Keywords: gastric cancer; long non-coding RNA taurine up-regulated gene 1; alkB homolog 5; proliferation; migration; angiogenesis
- From: Journal of Clinical Medicine in Practice 2024;28(12):66-71
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of long non-coding RNA (lncRNA) taurine up-regulated gene 1 (TUG1) on the proliferation, migration, and angiogenesis of gastric cancer cells. Methods The expression levels of LncRNA TUG1 in gastric cancer tissues and adjacent non-cancerous tissues were analyzed based on public databases. The expression of lncRNA TUG1 in gastric cancer cell lines was detected by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). SGC-7901 gastric cancer cells were transfected with si-TUG1 and si-NC, and the effects of knocking down lncRNA TUG1 on cell proliferation, colony formation, migration, and angiogenesis were analyzed using the Cell Counting Kit-8 (CCK-8) assay, colony formation assay, Transwell assay, and Matrigel tube formation assay. The correlation between alkB homolog 5 (
ALKBH5 ) gene and lncRNA TUG1 was analyzed based on public databases. The regulatory relationship betweenALKBH5 and lncRNA TUG1 was verified using Actinomycin D experiments. The effects of knocking downALKBH5 on the proliferation, colony formation, migration, and angiogenesis of gastric cancer cells were analyzed through cell function experiments. Results LncRNA TUG1 was up-regulated in gastric cancer tissues and cell lines. After knocking down lncRNA TUG1, the proliferation, colony formation, migration, and angiogenesis abilities of SGC-7901 cells were lower than those of control cells(P < 0.05). Database analysis results showed thatALKBH5 was positively correlated with lncRNA TUG1 expression in gastric cancer (r =0.37,P < 0.05). Compared with control cells, the RNA stability of lncRNA TUG1 in SGC-7901 cells with knocked-downALKBH5 decreased, and the cell proliferation, colony formation, migration, and angiogenesis abilities were also reduced (P < 0.05). ConclusionALKBH5 promotes the proliferation, colony formation, migration, and angiogenesis of gastric cancer cells by inducing the expression of lncRNA TUG1.
