Lipidomics analysis of medical Marmota himalayana oil
- VernacularTitle:医用喜马拉雅旱獭油的脂质组学分析
- Author:
Qin SU
1
;
Hailin WEI
;
Guohua ZHOU
;
Ling ZHANG
;
Pinghu ZHANG
Author Information
- Publication Type:Research Article
- Keywords:
gas chromatography-mass spectrometry;
ultra-high performance liquid chromatography-mass spectrometry;
Marmota himalayana oil; lipidomics; fatty acid - From: Journal of Clinical Medicine in Practice 2024;28(12):21-25
- CountryChina
- Language:Chinese
-
Abstract:
Objective To conduct lipidomics analysis of medical
Marmota himalayana oil and explore its potential biological effects. MethodsMarmota himalayana abdominal fat was heated and refined to produce medical marmot oil. Lipidomics analysis of two batches ofMarmota himalayana oil was performed using gas chromatography-mass spectrometry (GC-MS) and ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS). The potential effects ofMarmota himalayana oil were analyzed based on the results of lipid composition detection. Results GC-MS results showed that the main components of the two batches ofMarmota himalayana oil were saturated fatty acids (volume fraction of about 60%) and unsaturated fatty acids (volume fraction of about 30%). The saturated fatty acids were mainly palmitic acid, stearic acid, and arachidic acid, while the unsaturated fatty acids were mainly oleic acid and linoleic acid. There were no significant differences in the volume fraction of various fatty acids between the two batches, indicating that the preparation process ofMarmota himalayana oil was stable. The results of UPLC-MS negative ion mode detection showed thatMarmota himalayana oil contained 15 saturated fatty acids and 18 unsaturated fatty acids, but there were significant differences in volume fraction between the two batches. The results of UPLC-MS positive ion mode detection showed thatMarmota himalayana oil of both batches contained 15 diglycerides and 30 triglycerides. Conclusion This study innovatively applies GC-MS and UPLC-MS techniques to analyze the lipidomic components of medicalMarmota himalayana oil, providing a reliable basis for subsequent exploration of its pharmacological activity and the establishment of strict quality control standards.
