Whole-genome sequencing of SARS-CoV-2 from residual viral RNA present on positive rapid antigen test kits for genomic surveillance
- Author:
Mohd Ishtiaq Anasir
1
;
Khayri Kamel
2
;
Nor Malizza G Adypatti
1
;
Mohammad Syafiq Jamaluddin
1
;
Farah Amira Ahmad
1
;
Siti Nurhidayah Norhisham
1
;
Muhammad Zulfazli Mohamad Sukri
1
;
Nur Rafiqah Rosli
1
;
Siti Norazrina Saif
1
;
Nurul Izzati Basarudin
1
;
Mohamad Azzam-Sayuti
1
;
Akmal Hayat Abdul Karim
3
;
Mahirah Kamil Puat
3
;
Ravindran Thayan
1
;
Rozainanee Mohd Zain
1
Author Information
- Publication Type:Journal Article
- Keywords: COVID-19, whole genome sequencing, public health surveillance, diagnostic tests
- From: Western Pacific Surveillance and Response 2025;16(1):06-11
- CountryWHO-WPRO
- Language:English
- Abstract: This report describes an approach to recover SARS-CoV-2 RNA from rapid antigen test kit (RTK-antigen) cassettes for whole-genome sequencing (WGS). RNA samples were recovered from 33 RTK-antigen cassettes for WGS, with 18 samples achieving more than 80% genome coverage. This work provides a proof-of-concept that positive RTK-antigen cassettes can be safely transported, stored and subjected to WGS, enabling swift identification of circulating variants.
- Full text:2025041010073766817wpsar-16-1140 Anasir FINAL.pdf
