Influence of hybridization probe capture and amplicon library construction methods on HLA genotyping resolution level
10.13303/j.cjbt.issn.1004-549x.2025.03.001
- VernacularTitle:杂交探针捕获与扩增子文库构建法对HLA基因分型分辨水平的影响
- Author:
Xiaoni YUAN
1
;
Tengteng ZHANG
1
;
Yang LI
1
;
Xue JIANG
1
;
Tianjie YANG
1
;
Xiaojing BAO
1
;
Jun HE
1
Author Information
1. The First Affiliated Hospital of Soochow University, Jiangsu Provincial Institute of Hematology, Suzhou 215006, China
- Publication Type:Journal Article
- Keywords:
HLA;
next generation sequencing;
allele;
clinical application
- From:
Chinese Journal of Blood Transfusion
2025;38(3):303-308
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To compare next generation sequencing (NGS) library construction technology between probe hybridization capture and amplicon methods, and analyze the influencing factors of HLA genotyping resolution level and its prospects in clinical applications. [Methods] A total of 207 clinical samples with known typing results and samples from the proficiency testing plan were selected. The conformity rate of HLA genotyping results, allele coverage and typing data analysis indicators were confirmed, and the effects of two library construction methods on the level of HLA genotyping discrimination were compared. [Results] The concordance rate of 207 samples with the feedback results of PT or prior well-characterized HLA genotypes was 100%. Among them, 91 samples were captured using hybridization probe capture method. Compared with the original amplicon method, the hybridization probe capture method can distinguish the alleles of DRB1 and DPB1 that cannot be determined in 13 samples. The allelic imbalance of DRB1, DPA1, and DQB1 loci in 6 samples was resolved. Three samples were found to have missed detection of alleles at the DQA1 and DQB1 loci. [Conclusion] The performance indicators of hybridization probe capture and amplicon performance confirmation meet the requirements of clinical detection of HLA genotyping, which provides an experimental method and basis for clinical application.
