In Vitro and in vivo Component Identification of Danshenyin Based on UPLC-Q-TOF-MS/MS
10.13422/j.cnki.syfjx.20250766
- VernacularTitle:基于UPLC-Q-TOF-MS/MS的丹参饮体内外成分鉴定
- Author:
Sitong ZHANG
1
;
Xianrun HU
1
;
Wenkang LIU
1
;
Jinchun LEI
1
;
Xuemei CHENG
1
;
Xiaojun WU
1
;
Wansheng CHEN
1
;
Manlin LI
1
;
Changhong WANG
1
Author Information
1. Key Laboratory for Standardization of Chinese Medicines，Ministry of Education， Shanghai Key Laboratory for Compound Chinese Medicines，Institute of Chinese Materia Medica， Shanghai University of Traditional Chinese Medicine，Shanghai 201203，China
- Publication Type:Journal Article
- Keywords:
Danshenyin;
chemical constituent;
serum pharmacochemistry;
metabolites;
fragmentation pattern;
ultra performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry（UPLC-Q-TOF-MS/MS）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2025;31(9):175-183
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo elucidate the chemical composition of Danshenyin and its blood components in rats after oral administration. MethodsUltra performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry（UPLC-Q-TOF-MS/MS） coupled with PeakView 1.2 software was used to systematically characterize and identify the components of Danshenyin aqueous extract and its migratory components in rat blood after oral administration based on the retention time， quasi-molecular ion peaks， secondary fragmentation ions， and literature reports， and a preliminary compounds identification of Salviae Miltiorrhizae Radix et Rhizoma aqueous extract， the co-decoction of Santali Albi Lignum and Amomi Fructus was carried out to attribute the chemical constituents of the aqueous extract of Danshenyin. ResultsA total of 73 compounds， including 21 phenolic acids， 23 diterpenes， 6 flavonoids， 7 organic acids， 3 volatile oils and 13 others， were identified from the aqueous extract of Danshenyin. And 36 prototypes and 15 metabolites were identified in rat plasma， the major metabolic pathways included reduction， hydration， hydroxylation， demethylation， methylation， sulfation and others， these metabolites were mainly derived from tanshinones and salvianolic acids. ConclusionThe main blood components of the aqueous extract of Danshenyin are salvianolic acids and tanshinones， which may be the material basis of the efficacy. This study can provide reference for pharmacological research， quality control， and clinical application of Danshenyin.
