Analysis of main pharmacodynamic components of self-made activated prothrombin complex concentrate
10.13200/j.cnki.cjb.004437
- VernacularTitle:自制活化凝血酶原复合物的主要药效成分分析
- Author:
HUANG Yuwei
- Publication Type:Journal Article
- Keywords:
Activated prothrombin complex concentrate(aPCC);
Prothrombin complex concentrate(PCC);
FⅧ bypassing activity;
Patients with inhibitor
- From:
Chinese Journal of Biologicals
2025;38(03):312-317
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the composition of the pharmacodynamic components in the activated prothrombin complex concentrate(aPCC) developed by our research group,and to study the relationship between each pharmacodynamic component and the coagulation factor Ⅷ(FⅧ) bypassing activity.Methods The self-made aPCC concentrates were used as the research object,and the FⅧ bypassing activity was firstly detected by the coagulation method,and then the activities of a series of non-activated coagulation factors,activated coagulation factors and anti-coagulation factors were detected by the coagulation method and chromogenic substrate method.The prothrombin complex concentrate(PCC) in the market was used as a control to analyze the correlation between each pharmacodynamic component and FⅧ bypassing activity.Results The FⅧ bypassing activity of self-made aPCC concentrates and control PCC products were(43.53±3.07) IU/mL and(0.10 ±0.02) IU/mL,respectively,with a significant difference(t=20.16,P <0.01).Compared with the control PCC products,in the non-activated coagulation factors of self-made aPCC concentrates,the activities of coagulation factor Ⅶ(FⅦ) and coagulation factor Ⅸ(FⅨ) were significantly different(t=22.72 and 8.00,respectively,each P <0.05),and the activities of coagulation factor Ⅱ(FⅡ) and coagulation factor Ⅹ(F Ⅹ) showed no significant difference(t=1.67 and-0.96,respectively,each P> 0.05);in the activated coagulation factors of self-made aPCC concentrates,the activities of activated coagulation factor Ⅱ(FⅡ a),activated coagulation factor Ⅸ(FⅨ a),and activated coagulation factor Ⅹ(F Ⅹ a) were significantly different(t=15.92,32.93 and 34.64,respectively,each P <0.01),and there was no significant difference in the activity of activated coagulation factor Ⅶ(FⅦa)(t=2.34,P> 0.05);in the anti-coagulation factors of self-made aPCC concentrates,there was a significant difference in protein S(PS) activity(t=12.82,P <0.01),and there was no significant difference in heparin content or protein C(PC) activity(t=0.85 and-0.34,respectively,each P> 0.05).The FⅧ bypassing activity in the self-made aPCC concentrates and PCC products was significantly correlated with the activities of F Ⅶ,FⅨ,F Ⅱ a,FⅨ a,FⅩa and PS(r=0.999,0.971,0.988,0.994,0.974,and-0.984,respectively,each P <0.01),and was correlated with F Ⅹ a/F Ⅱ(r=0.827,P <0.05).Conclusion Self-made aPCC concentrates contain higher FⅦ bypassing activity,which may be related to the high activity of F Ⅶ,FⅨ,F Ⅱ a,FIX a and FⅩa.
