Immunogenicity of cell-based quadrivalent influenza subunit vaccine with nano-emulsion adjuvant in different age groups of BALB/c mice
10.13200/j.cnki.cjb.004434
- VernacularTitle:细胞基质四价流感病毒亚单位疫苗配伍纳米乳佐剂在不同鼠龄段BALB/c小鼠体内的免疫原性
- Author:
JIA Zheng
- Publication Type:Journal Article
- Keywords:
Quadrivalent influenza subunit vaccine;
Nano-emulsion adjuvant(NE);
Mice;
Mouse age;
Immunogenicity
- From:
Chinese Journal of Biologicals
2025;38(03):264-271+278
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of cell-based quadrivalent influenza subunit vaccine(QIVc) with nano-emulsion adjuvant(NE) on the enhancement of humoral immunity in mice.Methods Three-week-old,six-week-old and 18-month-old BALB/c female mice were immunized with QIVc combined with NE(50 μL) by intramuscular injection at the hemagglutinin(HA) dose of 1.5 μg(plant·unit),for two injections with the interval of 28 d.The hemagglutination inhibition(HI) titers and IgG antibody levels in serum of mice were measured 28 d after the initial immunization and 14 d after the last immunization,and the adjuvant enhancement effects of three age groups of mice were analyzed.The adjuvant-free and PBS control groups were set up.Fourteen days after the last immunization,the mice were challenged with 10 folds of median lethal dose(LD_(50)) B/Maryland/15/2016(B-Victoria) influenza strain,and the changes of body mass and survival rate were observed for 14 consecutive days.Results Intramuscular injection of HA combined with NE induced higher levels of serum HI and IgG antibodies than intramuscular injection of HA alone,and the immune-enhancing effect was more obvious in 18-month-old mice.NE enhanced the immunogenicity of HA and the resistance of mice to the attack of B/Maryland/15/2016(B-Victoria) influenza strain.Conclusion NE can enhance the immunogenicity of influenza antigen,and the enhancement effect on elderly mice is more obvious.
