Effect of bovine lactoferrin on proliferation and apoptosis of lung cancer A549 cells and its mechanism
10.13200/j.cnki.cjb.004418
- VernacularTitle:牛乳铁蛋白对肺癌A549细胞增殖和凋亡的影响及其机制
- Author:
SU Jinxian
- Publication Type:Journal Article
- Keywords:
Bovine lactoferrin(bLF);
Lung cancer;
Proliferation;
Apoptosis
- From:
Chinese Journal of Biologicals
2025;38(02):149-154
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of bovine lactoferrin(bLF) on the proliferation and apoptosis of lung cancer A549 cells and its possible mechanism, so as to provide an experimental basis for the study of anti-lung cancer effect of bLF.Methods A549 cells were treated with different concentrations of bLF. The effect of bLF on cell morphology was observed under microscope, the effect on cell activity was detected by CCK-8, the effects on cell proliferation and migration were verified by clone formation and scratch assay, and the expression of apoptosis-related genes and proteins was determined by qRTPCR and Western blot.Results The bLF had a significant inhibitory effect on the growth of A549 cells in a concentrationand time-dependent manner. Compared with the 0 mg/mL bLF group, the colony formation ability of A549 cells treated with0. 5 and 1. 0 mg/mL bLF significantly decreased(t = 6. 016 and 30. 24, P = 0. 003 8 and 0. 000, respectively), and the in vitro migration ability was gradually weakened. In addition, bLF induced the activation of apoptosis signaling pathway in A549cells. Compared with the control group, the expression levels of pro-apoptotic proteins Bax(t = 6. 454 and 11. 11, P = 0. 023 2and 0. 008, respectively) and P53(t = 6. 962 and 49. 46, P = 0. 002 2 and 0. 000 4, respectively) significantly increased and the expression level of anti-apoptotic protein Bcl-2(t = 4. 333 and 10. 44, P = 0. 049 4 and 0. 009 1, respectively) significantly decreased in the 0. 5 and 1. 0 mg/mL bLF groups. The bLF at a high concentration of 1. 0 mg/mL bLF group could cleave pro-caspase-3(t = 5. 759, P = 0. 028 9) signaling and activate caspase-3.Conclusion This study showed that bLF can significantlyinhibittheproliferationofA549lungcancercellsandpromotetheapoptosis,thusexertingananti-cancereffect.
