Whole gene sequence analysis of an echovirus 11 isolate from Kunming, Yunnan Province in 2019
10.13200/j.cnki.cjb.004395
- VernacularTitle:2019年云南省昆明市1株埃可病毒11型分离株的全基因序列分析
- Author:
LIU Yuhan
- Publication Type:Journal Article
- Keywords:
Enterovirus(EV);
Echovirus 11(E-11);
Whole genome;
Sequence analysis
- From:
Chinese Journal of Biologicals
2025;38(1):48-52
- CountryChina
- Language:Chinese
-
Abstract:
Objective To sequence the whole genome and analyze the genetic characteristics of an echovirus 11(E-11)strain isolated from stool samples of patients with hand, foot and mouth disease(HFMD) in Kunming, Yunnan Province in2019, so as to provide a reference for the prevention and control of E-11.Methods The virus was isolated using Vero cells,the RNA from cytopathic products was extracted for RT-PCR and sequenced, and the whole genome sequence was analyzed by software such as MEGA 7.0, Geneious 10.0 and Simplot 3.5.1.Results The 19V30322/YN/CHN/2019 isolate was sequenced and determined to be E-11, which belongs to the D5 subtype of the same genotype D as the E-11 epidemic strain in Guangdong and Hubei, China in recent years, with a full length of 7 434 nt and encoding a polyprotein of 2 195 amino acids. 19V30322 showed 81. 8%-98. 3% nucleotide identity and 94. 5%-99. 3% amino acid similarity with the domestic isolate E-11, and recombined with Coxsackievirus A10, E-6, Coxsackievirus B4 and Coxsackievirus B5 during the evolutionary process.Conclusion The 19V3/YN/CHN/2019 isolate is the D5 subtype of E-11 and is a recombinant strain.
