Establishment and evaluation of pendulum-like modified rat abdominal heart heterotopic transplantation model
10.12464/j.issn.1674-7445.2024251
- VernacularTitle:“钟摆式”大鼠腹部心脏异位移植改良模型的建立与评估
- Author:
Hongtao TANG
1
;
Caihan LI
2
;
Xiangyun ZHENG
2
;
Senlin HOU
2
;
Weiyang CHEN
2
;
Zengwei YU
2
;
Yabo WANG
1
;
Dong TIAN
2
;
Qi AN
1
Author Information
1. Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu 610041, China.
2. .
- Publication Type:OriginalArticle
- Keywords:
Heart transplantation;
Heterotopic transplantation;
Pendulum-like;
Modified;
Animal model;
Rat;
Donor heart procurement;
Donor heart perfusion
- From:
Organ Transplantation
2025;16(2):280-287
- CountryChina
- Language:Chinese
-
Abstract:
Objective To introduce the modeling method of pendulum-like modified rat abdominal heart heterotopic transplantation model and evaluate the quality of the model. Methods An operator without transplantation experience performed 15 consecutive models, recorded the time of each step, changes in body weight and modified Stanford scores, and calculated the surgical success rate, postoperative 1-week survival rate and technical success rate. Ultrasound examinations was performed in 1 week postoperatively. Results The times for donor heart acquisition, donor heart processing, recipient preparation and transplantation anastomosis were (14.3±1.4) min, (3.5±0.6) min, (13.6±2.1) min and (38.3±5.2) min respectively. The surgical success rate was 87% (13/15), and the survival rate 1 week after operative was 100% (13/13). The improved Stanford score indicated a technical success rate of 92% (12/13), and the postoperative 1-week ultrasound examination showed that grafts with Stanford scores ≥3 had detectable pulsation and blood flow signals. Conclusions The pendulum-like modified rat abdominal heart heterotopic transplantation improved model further optimizes the operational steps with a high success rate and stable quality, may be chosen as a modeling option for basic research in heart transplantation in the future.
