Construction and validation of a platelet-specific Metrnl gene knockout mouse model
10.12206/j.issn.2097-2024.202409031
- VernacularTitle:血小板特异性Metrnl基因敲除小鼠模型的构建与验证
- Author:
Canxin CHEN
1
,
2
;
Zhuwei MIAO
3
;
Chaoyu MIAO
3
Author Information
1. Department of Pharmacology, Naval Medical University, Shanghai 200433, China
2. Department of Hematology, The 910th Hospital of the Chinese People’s Liberation Army Joint Logistic Support Force, Quanzhou 362000, China.
3. Department of Pharmacology, Naval Medical University, Shanghai 200433, China.
- Publication Type:Originalarticles
- Keywords:
Metrnl;
gene-knockout mouse;
platelet
- From:
Journal of Pharmaceutical Practice and Service
2025;43(3):117-123
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the platelet-specific Metrnl gene knockout （Plt-Metrnl-/-）mice model. Methods Based on the Cre-LoxP system, Metrnlloxp/loxp mice, previously constructed in our laboratory, were mated with Pf4-Cre mice to generate Plt-Metrnl-/- mice. The genotypes of the offspring were identified, and tissues of the platelet, other peripheral blood cells, heart, liver, spleen, lung, kidney, brain, colon, and bone marrow were collected. The expression of the Metrnl gene in Plt-Metrnl-/- mice was investigated by quantitative real-time PCR and western blot. Also, the blood routine index was tested in Plt-Metrnl-/- mice. Results Compared with wild-type mice, the level of Metrnl protein in platelets was significantly decreased in Plt-Metrnl-/- mice. There was no significant difference in mRNA and protein levels of other peripheral blood cells and tissues, as well as in blood routine index, growth, and development between Plt-Metrnl-/- mice and WT mice. Conclusion Platelet-specific Metrnl knockout mice（Plt-Metrnl-/- mice）model was successfully constructed.
