Network Pharmacology Analysis on Mechanism Study of Buyang Huanwu Decoction for"Treating Different Diseases with Same Therapies"in Type 2 Diabetes Mellitus and Alzheimer's Disease
10.19378/j.issn.1003-9783.2024.09.010
- VernacularTitle:补阳还五汤"异病同治"2型糖尿病和阿尔兹海默症作用机制的网络药理学分析
- Author:
Hui XUE
1
,
2
;
Yanming XU
;
Jing JIANG
;
Xuetong MENG
;
Shumeng LIU
;
Qian ZHOU
;
Xia LEI
;
Ning ZHANG
Author Information
1. 黑龙江中医药大学药学院,黑龙江哈尔滨 150040
2. 黑龙江中医药大学佳木斯学院,黑龙江 佳木斯 154007
- Keywords:
Buyang Huanwu Decoction;
type 2 diabetes mellitus;
Alzheimer's disease;
treating different diseases with the same therapies;
network pharmacology;
molecular docking
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2024;35(9):1364-1375
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of Buyang Huanwu Decoction for"treating different diseases with same therapies"in type 2 diabetes mellitus(T2DM)and Alzheimer's disease(AD)based on network pharmacology and molecular docking techniques.Methods Firstly,the active ingredients of seven herbs in Buyang Huanwu Decoction were searched and screened by TCMSP,SymMap and other databases,the target prediction of these active ingredients was carried out by PharmMapper.The disease targets of T2DM and AD were collected from OMIM,DrugBank,GeneCards and Disgenet databases.The potential targets of Buyang Huanwu Decoction for"treating different diseases with same therapies"in T2DM and AD were obtained by intersecting with targets of active ingredients and the disease targets.Then STRING database and Cytoscape software were used to construct the PPI network and"herbs-components-targets"network,respectively.The core targets and pharmacodynamic components were screened through network topology analysis.Furthermore,GO functional and KEGG enrichment analysis was performed for potential targets using Metascape database.Finally,AutoDock software was used to verify the molecular docking between the selected components and targets.Results Ninety-four active components of Buyang Huanwu Decoction can act on 342 protein targets,and 100 intersection targets were obtained by comparing with 3 140 AD targets and 1 708 T2DM targets.GO functional enrichment analysis showed that these targets were mainly involved in MAPK cascade-mediated regulation,hormone-mediated signaling pathways,cellular response to lipids,regulation of inflammation response and other biological processes.MAPK,PI3K/Akt,FoxO,AGE/RAGE,insulin resistance,lipid and atherosclerosis,and non-alcoholic fatty liver signaling pathway were significantly enriched in KEGG analysis.PPI and topology analysis of"herbs-components-targets"network were used to screen out 10 core targets such as MAPK8,MAPK14,GSK3B,PPARG,and 10 core pharmacodynamic components such as paeoniflorin,benzoyl paeoniflorin,(+)-catechin.The results of molecular docking showed that these components had strong binding ability to the targets.Conclusion The core components of Buyang Huanwu Decoction,such as paeoniflorin and catechin,may act on PPARG,GSK3B and other key targets,and participate in the regulation of signaling pathways including MAPK and PI3K/Akt,which play a role in"treating different diseases with the same therapies"of T2DM and AD.
