Establishment of Bi-directional Solid Fermentation Process for Zhizhen Recipe and Evaluation of Its Efficacy
10.19378/j.issn.1003-9783.2024.07.015
- VernacularTitle:至真方固体双向发酵工艺的建立及其药效评价
- Author:
Yufei XIA
1
;
Songpo WANG
;
Zhong LIU
Author Information
1. 上海交通大学药学院,上海 200240
- Keywords:
Zhizhen Recipe;
fermentation process;
bi-directional solid fermentation;
anti-tumor activity;
anti-inflammatory activity;
pro-immune activity
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2024;35(7):1061-1068
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a new fermentation process for Zhizhen Recipe by using bi-directional solid fermentation method of traditional Chinese medicine.Methods ① Candidate strains for fermentation were primarily recruited through literature research.Then plate incorporation assay of candidate strains were conducted to investigate the growth compatibility between the strains.CCK-8 cell proliferation assay was used to compare the half proliferation inhibitory concentration of the products obtained under different fermentation conditions against human colorectal cancer cell line HCT-8.The advantages of different fermentation systems were evaluated and the key fermentation parameters including strains and incubation periods were determined,which gave rise to the establishment of the optimal fermentation process for Zhizhen Recipe.② In vitro pharmacological effects were compared between the fermented and unfermented Zhizhen Recipe by measuring proliferation inhibition of HCT-8 cells,as well as NO release and phagocytic activity of RAW264.7 cells.Results The results showed that Aspergillus niger/Saccharomyces cerevisiae was the optimal combination of strains,and the optimal fermentation time is 4 days.Under optimized conditions,the half inhibitory concentration(IC50)of the fermentation group on HCT-8 cells was 67.41 μg·mL-1,while IC50 value of the unfermented Zhizhen Recipe was 1 032 μg·mL-1.Compared with unfermented preparation,the fermented preparation exhibited significant effects on inhibiting the release of inflammatory factor NO of macrophages RAW264.7 cells and obviously enhanced their phagocytic activity.Conclusion In this present study,we established a new bi-directional solid fermentation process for Zhizhen Recipe.Under this fermentation system,the anti-colorectal cancer activity,anti-inflammatory activity and pro-immune activity of Zhizhen Recipe in vitro were significantly improved.
