Simultaneous Determination of Five Nucleoside Components in Periplaneta Americana by Quantitative Analysis of Multi-components by Single Marker Method
10.19378/j.issn.1003-9783.2024.07.014
- VernacularTitle:一测多评法测定美洲大蠊药材5种核苷类成分
- Author:
Muyuan CHEN
1
,
2
;
Siqi FENG
;
Zhenwen QIU
;
Qian LUO
;
Chunzhu XIE
;
Shiyu QI
;
Yuechun HUANG
;
Huibiao LI
Author Information
1. 广州中医药大学第一附属医院,广东 广州 510405
2. 广东省中医临床研究院,广东 广州 510405
- Keywords:
Periplaneta americana;
nucleosides;
uridine;
HPLC;
quantitative analysis of multi-components by single marker
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2024;35(7):1055-1060
- CountryChina
- Language:Chinese
-
Abstract:
Objective A HPLC-quantitative analysis of multi-components by single marker(QAMS)was established to determine 5 ingredients including uracil,uridine,hypoxanthine,inosine and guanosine in Periplaneta americana.Methods Separation took place on a Agilent ZORBAX SB-Aq column(250 mm×4.6 mm,5 μm)by gradient elution of methanol-0.01 mol·L-1 potassium dihydrogen phosphate at 20℃with a flow rate of 1.0 mL·min-1.The detection wavelength was 260 nm and the injection amount was 10 μL.The relative correction factors(fa/b)was calculated for the other four components with uridine as an internal standard.The content of 5 ingredients in 10 batches of Periplaneta americana was determined by QAMS.Results were compared with those of external standard method(ESM).Results Five nucleosides showed good linear relationships in their own ranges(r＞0.999 5),and the average recoveries ranged from 97.0%to 100.8%.The relative correction factors of uracil,hypoxanthine,inosine and guanosine were 0.908 0,1.005 3,1.969 5 and 1.303 4,respectively.Conclusion The established method is accurate and stable.It can provide theoretical reference for the quality control of Periplaneta americana.
