Application of Extract Reference Substance in Quality Analysis of Ginseng Formula Granules
10.19378/j.issn.1003-9783.2024.05.014
- VernacularTitle:对照提取物在人参配方颗粒质量分析中的应用
- Author:
Haiyan CHEN
1
;
Yin WU
;
Jingxian CHEN
;
Shaojuan WU
;
Yiyao ZHAGN
;
Fei FENG
;
Fei LIU
;
Shuang GAO
;
Longgang GUO
Author Information
1. 广州科曼生物科技有限公司,广东 广州 510663
- Keywords:
Ginseng Formula Granules;
extractive reference substance;
TLC;
HPLC;
quality analysis
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2024;35(5):725-729
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the quality of 19 batches of Ginseng Formula Granules from 11 different manufacturers by using Ginseng water Extract reference substance(GWERS)as references.Methods Thin layer chromatography(TLC)identification and feature map detection were carried out according to the identification items and characteristic maps of Ginseng Formula Granules standard(YBZ-PFKL-2021186)issued by the National Medical Products Administration.Results The results of TLC analysis showed that the 19 batches of Ginseng Formula Granules-labeled samples could be divided into three categories.The overall pattern of the first type of samples was consistent with that of GWERS,and the similarity was high.Pseudoginsenoside F11,a unique component of American ginseng,was detected in the second type of samples.Four blue fluorescent spots were observed in the third type of samples compared to GWERS.HPLC results indicated that all 19 batches of Ginseng Formula Granules showed eight characteristic peaks at the same retention time as that of GWERS chromatogram.Two more chromatographic peaks were found in the chromatogram of three batches of samples from one manufacturer compared to the chromatogram of other samples,whose similarity to the GWERS chromatogram was less than 0.65.The similarity between the chromatogram of the remaining 16 sample and GWERS chromatogram was higher than 0.94.Conclusion At present,the quality of Ginseng Formula Granules on the market varies greatly.It was suspected that American ginseng might appear among them.The application of GWERS for quality analysis of Ginseng Formula Granules has better applicability to the control medicinal materials.
