Exploration of the Pharmacological Substance Basis and Action Characteristics of Wenxing Jingjintong Gel Patch in the Treatment of Rheumatoid Arthritis Based on System Pharmacology and LC-MS/MS Technology
10.19378/j.issn.1003-9783.2024.05.012
- VernacularTitle:基于系统药理学结合LC-MS/MS技术探讨温性经筋通贴膏治疗类风湿性关节炎的药效物质基础与作用特点
- Author:
Anyu SU
1
;
Weibin HUANG
;
Jingyuan MO
;
Zixuan ZHANG
;
Xun XIE
;
Xiaobing HUANG
;
Bilian ZHONG
;
Yongping ZHANG
;
Lisheng WANG
;
Chunyi WANG
Author Information
1. 广州中医药大学中药学院,广东 广州 510006
- Keywords:
Wenxing Jingjintong Gel Patch;
rheumatoid arthritis;
LC-MS/MS;
system pharmacology;
pharmacological substance basis;
action characteristic
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2024;35(5):706-718
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the key active ingredients and action characteristics of Wenxing Jingjintong Gel Patch in the treatment of rheumatoid arthritis(RA)based on the systematic pharmacology and LC-MS/MS technology.Methods The information of active ingredient from Wenxing Jingjintong Gel Patch was established through LC-MS/MS analysis and literature retrieval.The targets of the active ingredients were predicted using Swiss Target Prediction platform and then mapped with the RA-related targets obtained from GeneCards,DrugBank,and OMIM databases to identify the intersecting targets.The"active ingredients-effective targets"network was constructed through the Cytoscape software.The shared targets were imported into STRING database to construct a protein-protein interaction network.GO function and KEGG pathway enrichment analysis were performed using the Metascape database.Molecular docking studies were conducted using AutoDock software to investigate the interactions between key ingredients and target proteins.Results A total of 142 active ingredients were identified in Wenxing Jingjintong Gel Patch by wsing LC-MS/MS,which were further supplemented to 174 through literature retrieval.There were 175 shared targets between the active ingredients and RA.It was anticipated that Wenxing Jingjintong Gel Patch exerted immune regulation and anti-inflammatory and analgesic effects through the interaction between key active ingredients such as berberine,neobavaisoflavone,and palmatine chloride with key targets,including TNF,IL6,and AKT1 to regulate PI3K/Akt1,JAK/STAT,and MAPK signaling pathways.In 1 152 molecular docking validation,94%of them had binding energies less than-5.0 kcal·mol-1,while 51%of them had binding energies less than-7.0 kcal·mol-1.It was indicated that there was a good binding affinity between the potential active ingredients and core targets.Conclusion This study predicted the active ingredients and action characteristics of Wenxing Jingjintong Gel Patch in the treatment of RA,which provided a theoretical basis for further clinical application and quality control.
