Mechanism of relieves insomnia effect of Jiaotai pills based on network pharmacology
10.3969/j.issn.1673-9701.2024.32.017
- VernacularTitle:基于网络药理学探讨交泰丸治疗失眠的作用机制
- Author:
Yifan LU
1
;
Zhengzhong YUAN
Author Information
1. 温州医科大学附属第一医院药学部,浙江温州 325000
- Keywords:
Jiaotai pills;
Insomnia;
Network pharmacology;
Molecular docking
- From:
China Modern Doctor
2024;62(32):77-81
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the potential mechanism of the relieves insomnia effect of Jiaotai pills by virtue of network pharmacology and molecular docking.Methods The active ingredients of Jiaotai pills are obtained by Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),Integrative Pharmacology-based Research Platform of Traditional Chinese Medicine(TCMIP).Related targets of Jiaotai pills were predicted by SwissTargetPrediction.The potential targets associated with insomnia were retrieved from DisGeNET,DrugBank.Venny 2.1 was employed to identify the common targets shared by active components,insomnia,and core nodes,and the key components and key targets were screened by further topological analysis.The key components and key targets were analyzed by the network analysis plug-in of Cytoscape 3.9.1 and the core components were screened out.The protein-protein interaction network and the core targets were built by STRING 12.0.Gene ontology(GO)functional annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment were performed in DAVID.AutoDockTools1.5.7 was used for the molecular docking between the key targets and the corresponding key components.Apply Pymol was used to visualize the results.Results A total of 5 active components of Jiaotai pills were screened,including Berberine,Limonin,Obacunone,Moupinamide,Palmatine,and core targets such as solute carrier family 6 member(SLC6A4),dopamine receptor D2(DRD2),cholinergic receptor nicotinic alpha 4 subunit(CHRNA4),tumor necrosis factor(TNF),and prostaglandin-endoperoxide synthase 2(PTGS2)were screened.The signaling pathways of Jiaotai pills for insomnia mainly concentrated in regulating 5-hydroxytryptaminergic synapses and dopamine synapses.Molecular docking showed that core components Berberine,Limonin,and Obacunone and core targets SLC6A4,DRD2,CHRNA4,TNF,and PTGS2 and so on were well docked.Conclusion Berlambine,Llimonin,Obacunone in Jiaotai pills through multiple mechanisms to treat insomnia by affecting the expression of SLC6A4,DRD2,TNF,CHRNA4,and PTGS2.
