Study on the preparation process and quality standards of Jiawei Suoquan mixture
10.3969/j.issn.1673-9701.2024.30.012
- VernacularTitle:加味缩泉合剂制备工艺及质量标准研究
- Author:
Lili MENG
1
;
Feixiang HUANG
;
Jianbing XU
Author Information
1. 杭州市妇产科医院药剂科,浙江杭州 310008
- Keywords:
Jiawei Suoquan mixture;
Preparation process;
Quality standard;
Content determination;
Morroniside;
Loganin
- From:
China Modern Doctor
2024;62(30):61-65,125
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the preparation process of Jiawei Suoquan mixture and determine the quality standard.Methods The preparation technology of Jiawei Suoquan mixture was determined by orthogonal test with the yield of paste,the total amount of morroniside and loganin as indexes.The column was Diamonsil Plus 5μm C18 (250mm×4.6mm).Acetonitrile as mobile phase A,0.3％ phosphoric acid solution as mobile phase B,gradient elution;The detection wavelength was 240nm;The flow rate was 1.0ml/min.The column temperature was 35℃.Results The determined extraction process conditions were extracted twice,with 10 times of water and 8 times of water,respectively,for 1.5h and 1.0h.The established methods for the determination of morroniside and loganin were specific,reproducibility and accurate.The linear relationship of morroniside was good in the range of 0.1962 to 0.9810μg,the average recovery was 100.83％,relative standard deviation (RSD) was 1.26％,and the precision and stability met the requirements.The linear relationship of loganin in the sample size range from 0.0995 to 0.7960μg was good.The average recovery was 97.10％ and RSD was 0.84％.The precision and stability met the requirements.Conclusion The preparation process of Jiawei Suoquan mixture is simple and feasible.The content determination method is specific,accurate and reliable,and can be used for quality control of Jiawei Suoquan mixture.
