Study on the role of fibroblast growth factor 21 in liver lipid deposition
10.3969/j.issn.1673-9701.2024.25.005
- VernacularTitle:成纤维细胞生长因子21在肝脏脂质沉积中的作用研究
- Author:
Pei PEI
1
;
Yijia FAN
;
Guoqin LU
;
Yiqun TENG
;
Feng ZHU
Author Information
1. 嘉兴大学附属第二医院儿童医学中心,浙江嘉兴 314000
- Keywords:
Fibroblast growth factor 21;
Lipid deposition;
Glutathione peroxidase 4
- From:
China Modern Doctor
2024;62(25):22-25
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of fibroblast growth factor(FGF)21 in liver lipid deposition.Methods HepG2 cells were divided into control group and induction group.Fatty liver cell model was constructed using oleic acid and palmitic acid in induction group.Lipid deposition under the microscope was observed between two groups by oil red O staining,and the protein expression of FGF21 and glutathione peroxidase 4(GPX4)in two groups were detected.FGF21 lentivirus-infected fatty liver cells were used as interference group,and scramble lentivirus-infected fatty liver cells were used as non-interference group.The expression level of FGF21 mRNA,lipid deposition and expression level of GPX4 protein were compared between two groups.Results Compared with control group,induction group had significant lipid deposition,significantly increased expression level of FGF21 protein,and significantly decreased expression level of GPX4 protein(P＜0.05).After the interference of FGF21 lentivirus,expression level of FGF21 mRNA was significantly decreased,lipid droplets were decreased,lipid deposition was significantly improved,and GPX4 protein expression level was significantly increased in interference group(P＜0.05).Conclusion FGF21 may promote liver lipid formation by inhibiting lipid peroxidation.
