Study on the correlation between neuropeptide S receptor gene polymorphism and primary insomnia
10.3969/j.issn.1673-9701.2024.21.001
- VernacularTitle:神经肽S受体基因多态性与原发性失眠的相关性研究
- Author:
Jie FAN
1
,
2
;
Peilin HUI
;
Wei MA
;
Xubin WANG
;
Yuan ZHAO
;
Jinfeng WANG
;
Xueping CHEN
;
Xiaoyan SU
;
Bin GUO
;
Yuping XIE
Author Information
1. 甘肃省人民医院睡眠医学中心,甘肃兰州 730000
2. 甘肃省睡眠临床医学研究中心,甘肃兰州 730000
- Keywords:
Neuropeptide S;
Primary insomnia;
Single nucleotide polymorphism
- From:
China Modern Doctor
2024;62(21):1-5,46
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between neuropeptide S receptor(NPSR)single nucleotide polymorphism(SNP)(rs323917,rs323920,rs323922,rs2530547,rs324957)and primary insomnia(PI).Methods A total of 157 patients diagnosed with PI in the outpatient department of Center of Sleep Medicine,Gansu Provincial People's Hospital from December 2016 to May 2019 were selected as PI group,and 133 healthy physical examination subjects during the same period were selected as control group.Venous blood samples were collected and DNA,polymerase chain reaction(PCR)amplification and agarose gel electrophoresis were extracted.rs323917,rs323920,rs323922,rs2530547,rs324957 single nucleotide loci were genotypized by target site sequencing.Meanwhile,standard polysomnosis monitoring was performed to analyze the correlation between gene polymorphism and PI.Results There were no significant differences in the genotype distribution of NPSR SNP sites(rs323917,rs323920,rs323922,rs2530547)and allele frequency and rs324957 allele frequency between two groups(P＞0.05).There was significant difference in genotype distribution of rs324957(P=0.034).There was no significant difference in the frequency of different haplotypes between two groups(P＞0.05).Conclusion The expression of rs324957 SNP genotypes in NPSR may be related to PI,and AG genotype is dominant.
