Evaluation of ZOPOMED-60 mycobacterium tuberculosis microscanner for detecting acid-fast bacilli in sputum smears
10.3969/j.issn.1673-9701.2024.16.007
- VernacularTitle:ZOPOMED-60结核分枝杆菌显微扫描仪检测抗酸杆菌效果
- Author:
Jie DONG
1
;
Liusheng TANG
;
Xiaobing YANG
;
Liling HUANG
Author Information
1. 广西壮族自治区胸科医院医学检验科,广西柳州 545005
- Keywords:
Microscanner;
Acid-fast bacilli;
Sensitivity;
Specificity;
Consistency
- From:
China Modern Doctor
2024;62(16):25-28
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of ZOPOMED-60 mycobacterium tuberculosis microscanner on detecting acid-fast bacilli in sputum smears.Methods A total of 203 qualified sputum specimens from patients treated in Chest Hospital of Guangxi Zhuang Autonomous Region in May 2023 were collected.The samples were stained by using Ziehl-Neelsen staining and fluorecent staining,respectively,and the acid-fast bacillus was detected by the manual microscopy and ZOPOMED-60 mycobacterium tuberculosis microscanner.The sensitivity,specificity,positive predictive value and negative predictive value of acid-fast bacillus were calculated using different methods based on liquid culture of mycobacterium.Results The positive rates of manual reading and instrument intelligent reading of fluorecent staining were 12.3%and 18.7%,respectively,and the positive rates of manual reading and instrument intelligent reading of Ziehl-Neelsen staining were 13.3%and 17.7%,respectively.There was no significant difference between the four reading results(P>0.05).Based on the results of liquid culture of mycobacterium,sensitivity of manual reading and instrument intelligent reading of fluorecent staining and manual reading and instrument intelligent reading of Ziehl-Neelsen staining were 40.0%,45.0%,45.0%and 53.3%,specificity were 99.3%,92.3%,100.0%and 97.2%,positive predictive values were 96.0%,71.0%,100.0%and 88.9%,and negative predictive values were 79.8%,80.0%,81.2%and 83.2%,respectively.The consistency was good.Conclusion The performance of ZOPOMED-60 mycobacterium tuberculosis microscanner in the detection of acid-fast bacilli by two staining methods is good,and it is worth popularizing.
