Analysis of the influence of novel coronavirus infection on HIV ELISA results
10.3969/j.issn.1673-9701.2024.14.015
- VernacularTitle:120例无偿献血者HIV-ELISA单试剂反应性结果的分析
- Author:
Lingli XU
1
;
Yujun GE
;
Jun XU
Author Information
1. 嘉兴市中心血站检验科,浙江嘉兴 314000
- Keywords:
Enzyme linked immunosorbent assay;
Chemiluminescence immunoassay;
Nucleic acid amplification test;
Voluntary blood donors
- From:
China Modern Doctor
2024;62(14):65-68
- CountryChina
- Language:Chinese
-
Abstract:
Objective Analyze and study the detection performance of single reagent reactivity results of human immunodeficiency virus antigen antibody enzyme-linked immunosorbent assay(HIV-ELISA)in unpaid blood donors,to provide reference for improving the laboratory's testing capabilities.Methods Detection of 7069 specimens from our station in February 2023 using the BR-HIV reagent,among them,there were 120 cases of BR-HIV single reagent reactivity,then,three different manufacturers of HIV-ELISA reagents were used for the single reagent reactivity sample,chemiluminescence immunoassay(CLIA),nucleic acid detection technology(NAT)and Western blot for detection,statistical analysis of the relationship between ELISA,CLIA,NAT and Western blot detection results.Results Phased BR-HIV single reagent reactivity increase is related to A virus A infection,the difference is statistically significant(χ2=4.058,P<0.05).Tracking the information of 120 specimens by phone,data analysis of BR-HIV single reagent reactive A virus A infected persons and uninfected persons S/CO value,infected individuals are significantly higher than uninfected individuals.Infected individuals are significantly higher than uninfected individuals,the difference is statistically significant(χ2=44.099,P<0.05).CLIA,NAT,and three other HIV-ELISA detection reagents have consistency(Kappa=1),with equivalence.The results of immunoblotting test(Western blot)were all negative.Conclusion The false positive rate of BR-HIV single reagent in the samples of unpaid blood donors who had been infected with A virus A increased,CLIA,NAT,and other enzyme exempt agents can be used instead of testing,reduce the scrapping of blood,improve the detection ability of blood.
