Expressions of miRNA-495-3P and Hillday cross-recognition protein in laryngeal carcinoma and their clinical significances
10.3760/cma.j.cn115355-20240305-00101
- VernacularTitle:miRNA-495-3p、Hillday交叉识别蛋白在喉癌组织中的表达及其临床意义
- Author:
Deshui YU
1
;
Liang WANG
Author Information
1. 郑州大学第一附属医院耳鼻咽喉头颈外科，郑州 450052
- Keywords:
Laryngeal neoplasms;
MicroRNAs;
Hillday cross-recognition protein
- From:
Cancer Research and Clinic
2024;36(9):641-645
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expressions and correlations of miRNA-495-3p (miR-495-3p) and Hillday cross-recognition protein (HJURP) in laryngeal carcinoma tissues, and their relationship with clinicopathological features of patients.Methods:Cancerous tissues and corresponding paracancerous tissues (more than 1 cm away from the boundary of cancerous tissue) were selected from 30 patients with primary laryngeal squamous cell carcinoma in the First Affiliated Hospital of Zhengzhou University from June to October 2023. Real-time fluorescence quantitative polymerase chain reaction was used to detect the relative expression of miR-495-3p. The relative expression of HJURP protein was detected by Western blotting. The correlation between miR-495-3p and HJURP in laryngeal carcinoma was analyzed by Pearson method.Results:Compared with paracancerous tissues, the relative expression of miR-495-3p in laryngeal cancer tissues was lower (0.28±0.06 vs. 1.00±0.00), and the difference was statistically significant ( t = -66.57, P < 0.001); the relative expression of HJURP protein in laryngeal carcinoma tissues was higher (10.2±5.6 vs. 1.0±0.0), and the difference was statistically significant ( t = 9.02, P < 0.001). The results of Pearson correlation analysis showed that miR-495-3p was negatively correlated with HJURP in laryngeal cancer tissues ( r = -0.63, P < 0.001). The relative expression of miR-495-3p in patients with lymph node metastasis was lower than that in patients without lymph node metastasis (0.23±0.03 vs. 0.30±0.05), and the difference was statistically significant ( t = -4.89, P < 0.001); the relative expression of HJURP protein was higher than that in patients without lymph node metastasis (13.6±5.1 vs. 8.8±5.3), and the difference was statistically significant ( t = 2.33, P = 0.030). The relative expression of miR-495-3p in patients with clinical stage Ⅲ-Ⅳ was lower than that in patients with stage Ⅰ-Ⅱ (0.23±0.03 vs. 0.32±0.04), and the difference was statistically significant ( t = 7.22, P < 0.001); the relative expression of HJURP protein was higher than that in patients with stage Ⅰ-Ⅱ (15.3±5.4 vs. 6.9±2.2), and the difference was statistically significant ( t = -5.85, P < 0.001). Conclusions:In laryngeal carcinoma, the expression of miR-495-3p is decreased, and the expression of HJURP protein is increased; the two are related.
