Effects of Luhong Formula on Myocardial Fibrosis and STAT3
10.16466/j.issn1005-5509.2024.09.003
- VernacularTitle:鹿红方抗心肌纤维化作用及对STAT3的影响
- Author:
Jijie XU
1
;
Jian DAI
;
Xiaohui LI
Author Information
1. 上海市宝山区中西医结合医院 上海 201999
- Keywords:
chronic heart failure;
myocardial infarction;
Luhong Formula;
myocardial fibrosis;
signal transduction and transcriptional activator 3;
pro-fibrotic factor;
heart-kidney Yang deficiency;
heart-kidney synergy therapy
- From:
Journal of Zhejiang Chinese Medical University
2024;48(9):1074-1082
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To study the mechanism of Luhong Formula in relieving myocardial fibrosis after myocardial infarction.[Methods]The myocardial fibrosis model after myocardial infarction was prepared by coronary artery ligation.Sixty SD rats were randomly assigned to sham operation group,model group,Luhong Formula group and Perindopril group.Each group completed the corresponding intervention for 4 weeks.Heart structure and left ventricular ejection fraction were measured by ultrasonic cardiogram.The pathological changes of cardiac fibrosis were observed by hematoxylin-eosin(HE)staining,Masson and Sirius red staining.The secretion of cardiac signal transduction and activator of transcription 3(STAT3)was analyzed by Western blot.Enzyme-linked immunosorbent assay(ELISA)was used to detect the serum levels of pro-fibrotic factor including connective tissue growth factor(CTGF),platelet reactive protein-1(TSP-1)and matrix metalloproteinase inhibitor-1(TIMP-1).[Results]Compared with model group,the intervention of Luhong Formula and Perindopril could inhibit the increase of left ventricular enddiastolic volume and left ventricular enddiastolic diameter,while the value of left ventricular ejection fraction was significantly increased(P<0.05).The effect of Luhong Formula group on improving ventricular dysdilation and increasing left ventricular ejection fraction was similar to that of Perindopril group.HE staining results showed that compared with model group,the number of abnormal cardiomyocytes in Luhong Formula group and Perindopril group was significantly reduced,the interstitial swelling was reduced,and the inflammatory cell infiltration was reduced.Masson and Sirius red staining showed that compared with model group,both Luhong Formula group and Perindopril group could reduce the degree of myocardial fibrosis after intervention.The effect of Luhong Formula group on improving the myocardial pathological changes and alleviating myocardial fibrosis was similar to that of Perindopril group.Western blot results showed that compared with model group,intervention in both Luhong Formula group and Perindopril group could significantly increase the myocardial STAT3 secretion(P<0.05),and the effect of Luhong Formula group on promoting myocardial STAT3 secretion was similar to that of Perindopril group(P>0.05).ELISA results showed that the levels of CTGF,TSP-1 and TIMP-1 in Luhong Formula group and Perindopril group were significantly lower than those in model group(P<0.05),and the inhibitory effects of CTGF,TSP-1 and TIMP-1 in Luhong Formula group were similar to those in Perindopril group(P>0.05).[Conclusion]Luhong Formula can significantly inhibit myocardial fibrosis after myocardial infarction,and its mechanism is related to up-regulating STAT3 level and inhibiting the secretion of pro-fibrotic factors CTGF,TSP-1 and TIMP-1.
