Study on the Effect of Effective Parts of Wild Chrysanthemum on Two Kinds of Hyperuricemic Animals and Safety Evaluation
10.16466/j.issn1005-5509.2024.09.002
- VernacularTitle:野菊花有效部位对两种高尿酸血症动物的作用研究及安全性评价
- Author:
Mengxi CHANG
1
;
Minxia PANG
;
Jie SU
Author Information
1. 浙江中医药大学药学院 杭州 310053
- Keywords:
effective parts of wild chrysanthemum;
hyperuricemia;
animal model;
serum;
uric acid;
liver function;
kidney function;
security
- From:
Journal of Zhejiang Chinese Medical University
2024;48(9):1063-1073
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the effects of effective parts of wild chrysanthemum(CYM.E)on potassium oxonate complex hypoxanthine-induced hyperuricemia in mice and high purine diet-induced hyperuricemia in rats and to evaluate the safety of CYM.E.[Methods](1)Drug safety experiments:Mice were divided into control group,allopurinol group(15 mg/kg),benzbromarone group(7.5 mg/kg),and CYM.E low,medium and high dose groups(30,60 and 90 mg/kg),and were administered by gavage for 2 weeks to evaluate the safety of CYM.E administration.(2)Experiments on hyperuricemia mice caused by hypoxanthine compounded with potassium oxonate:Mice were divided into control group,model group,allopurinol group(15 mg/kg),benzbromarone group(7.5 mg/kg),and CYM.E low,medium,and high dose groups(30,60 and 90 mg/kg),and administered by gavage for 1 week,and the serum levels of uric acid(UA),creatinine(CR),blood urea nitrogen(BUN),alanine aminotransferase(ALT)and aspartate aminotransferase(AST)were detected.(3)Experiments on hyperuricemia rats caused by a high-purine diet:The rats were divided into control group,model group,allopurinol group(10 mg/kg),benzbromarone group(5 mg/kg),and CYM.E low,medium,and high dose groups(15,30 and 60 mg/kg),and were administered by gavage for 5 weeks,and the serum levels of UA,CR,BUN,ALT and AST were detected in each group.[Results]CYM.E did not affect serum UA levels and liver and kidney function indices in normal mice in drug safety experiments.The experiments of potassium oxonate combined with hypoxanthine in hyperuricemic mice indicated the medium and high doses of CYM.E could significantly reduce the serum UA level of hyperuricemic mice(P<0.05,P<0.01).The experiment of rats with hyperuricemia caused by high-purine diet showed,after 3 weeks of administration,the high dose of CYM.E could significantly reduce serum UA level(P<0.01);after 5 weeks of administration,the medium and high doses of CYM.E could significantly reduce the serum UA level(P<0.05,P<0.01),and the high dose of CYM.E could significantly reduce the serum CR and BUN levels(P<0.01,P<0.05),and the medium dose could significantly reduce the serum AST level(P<0.05).[Conclusion]CYM.E did not affect the UA level of normal mice,and no related liver and kidney injury was seen;and after reaching a certain dose,it had a better uric acid-lowering effect on both models of hyperuricemia,and had a certain protective effect on liver and kidney functions.
