Soil Amendment Combined with Trichoderma Inoculum to Alleviate Atractylodes Macrocephala Replant Disease and Its Micro-ecology in the Rhizosphere
10.16466/j.issn1005-5509.2024.09.001
- VernacularTitle:土壤改良剂联合木霉菌液缓解白术连作障碍及其根际微生态研究
- Author:
Chenxian GU
1
;
Yujin ZHAO
;
Jingyan XU
Author Information
1. 浙江中医药大学生命科学学院 杭州 310053
- Keywords:
Atractylodes macrocephala;
rhizosphere;
microbial community;
soil amendment;
biological bacteria agent;
replant disease
- From:
Journal of Zhejiang Chinese Medical University
2024;48(9):1055-1062
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To explore the micro-ecological mechanism of soil amendment combined with Trichoderma solution in resolving the replant obstacles of Atractylodes macrocephala through studying their effects on the microbial community structure in the rhizosphere soil.[Methods]In pot experiment and field experiment,different soil amendments and Trichoderma inoculants were applied to Atractylodes macrocephala,the survival rate,physiochemical properties of soil and microbial community were detected,as well as analysis of the microenvironmental changes in the rhizosphere.[Results]The number of leaves and survival rate were significantly increased after the application of lime(mainly composed of calcium oxide)and mixed bacterial solution;the activity of soil-urease(S-UE),soil-phosphatase(S-NP),solid-β-glucosidase(S-β-GC),soil-catalase(S-CAT)in the rhizosphere were also increased,indicating that the replant obstacle was alleviated.Meanwhile,the rhizosphere bacteria and fungi were the highest.The sequencing results showed that the bacteria in the rhizosphere soil after the application of lime and mixed bacterial solution were mainly composed of Gemmatimonadetes,Verrucomicrobia,Firmicutes,Actinobacteria,while the fungi were mainly composed of Ascomycetes,Mortierella,Basidiomycetes and Chytridiomycota,after the application of lime and mixed bacterial solution,the proportion of Fusarium decreased,and the proportion of Penicillium increased.[Conclusion]The combination of lime as a soil amendment and Trichoderma can effectively improve the replant disorder of Atractylodes macrocephala and promote the growth of Atractylodes macrocephala,which may be by improving the structure of the microbial communities in the rhizosphere,increasing the abundance of biocontrol bacteria and reducing the abundance of pathogenic bacteria.
