Based on Intestinal flora-LPS/TLR4 to Research the Effect of Chrysanthemi Indici Flos Extract on the Model of Hyperuricemia Induced by Overeating Greasy
10.16466/j.issn1005-5509.2024.08.003
- VernacularTitle:基于肠道菌群-LPS/TLR4通路探讨野菊花提取物对"过食膏粱厚味"致高尿酸血症大鼠的作用
- Author:
Lin JIAO
1
;
Minxia PANG
;
Yingjie DONG
Author Information
1. 浙江中医药大学药学院 杭州 310053
- Keywords:
hyperuricemia;
fat emulsion;
Chrysanthemum indicum L.;
animal model;
lipid metabolism;
microinflammation;
intestinal microecology;
LPS/TLR4
- From:
Journal of Zhejiang Chinese Medical University
2024;48(8):915-928
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To study the effect and mechanism of Chrysanthemi Indici Flos extract(CYM.E)on hyperuricemia in rats induced by"overeating greasy".[Methods]Sixty-four rats were randomly divided into normal control group,model control group,allopurinol 10mg·kg-1 group,Benzbromarone 5 mg·kg-1 group,and CYM.E 15,30,60 and 90 mg·kg-1 group,with 8 rats in each group.Except for the normal control group,the other groups used fat emulsion to establish a rat model of anthropomorphic hyperuricemia based on"overeating greasy".During the experiment,serum uric acid(UA),total cholesterol(TC),triglyceride(TG),high density lipoprotein cholesterol(HDL-c)and low density lipoprotein cholesterol(LDL-c)levels were measured.Interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)and lipopolysaccharide(LPS)were determined by enzyme linked immunosorbent assay(ELISA).The mRNA expression of TNF-α and Toll like receptor 4(TLR4)in the small intestine was measured by Real-time quantitative polymerase chain reaction(qRT-PCR);and the protein expression of TLR4 in the small intestine was measured by Western blot.Intestinal microecology was classified with operational taxonomic units(OTU),and OTU was analyzed with structure,and LEfSe difference,etc.[Results]CYM.E significantly reduced the serum UA,TC,LDL-c,IL-6 and TNF-αlevels of model rats;and reduced plasma LPS level.Moreover,CYM.E could reduce the expression of TNF-α mRNA and TLR4 mRNA in the small intestine,and reduced the protein expression of TLR4 in the small intestine,thus improving the microinflammatory state of the intestinal tract,increased the abundance and species of Lactobacillales and Bifidobacteriales,and reduced the abundance and species of Bacteroidales and Escherichia coli.[Conclusion]CYM.E has the effects of decreasing serum UA levels,regulating blood lipids,decreasing inflammatory damage,and decreasing endotoxin release,which may exert an UA-lowering effect by regulating intestinal microecology.
