Study on the Effect of Polygonatum Sibiricum Red.on Immune Dysfunction of Middle-aged Rats with Kidney Essence Defi-ciency
10.16466/j.issn1005-5509.2024.08.001
- VernacularTitle:黄精改善中年房劳肾精亏虚大鼠免疫功能紊乱的作用研究
- Author:
Jingjing YU
1
;
Yuzhong DU
;
Jie SU
Author Information
1. 浙江中医药大学 杭州 310053
- Keywords:
Polygonatum sibiricum Red.;
middle-aged model with kidney essence deficiency caused by sexual overstrain;
immune dysfunction;
T lymphocyte;
proliferation;
apoptosis;
PI3K/AKT
- From:
Journal of Zhejiang Chinese Medical University
2024;48(8):895-904
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To study the regulatory effect of Polygonatum sibiricum Red.on immune dysfunction in middle-aged rats with kidney essence deficiency caused by sexual overstrain.[Methods]To simulate the immune dysfunction caused by kidney essence deficiency due to sexual overstrain in middle-aged men,middle-aged rats with kidney essence deficiency model caused by sexual overstrain were prepared by"middle-aged male rats+male and female with the same cage".The spleen coefficient of rats was measured during the experiment;the morphological changes of spleen were observed by hematoxylin-eosin(HE)staining;the proportion of neutrophils and lymphocytes in bone marrow was measured,and the number and proportion of lymphocytes in peripheral blood and the proportion of neutrophils were measured;splenic lymphocyte proliferation assay was used to detect splenic lymphocyte proliferation;the proportion of T lymphocyte subsets in spleen tissue was determined by flow cytometry;terminal deoxynucleoitidyl transferase mediated nick end labeling(TUNEL)was used to observe the apoptosis of spleen cells;Western blot was used to detect the protein expression of phospho-phosphatidylinositol 3-kinase(p-PI3K),phospho-protein kinase B(p-AKT),B-cell lymphoma-2(Bcl-2)and Bcl-2 associated X(Bax)in spleen tissue.[Results]The results showed that Polygonatum sibiricum Red.could reduce the proportion of neutrophils in bone marrow and peripheral blood of middle-aged rats with kidney essence deficiency caused by sexual overstrain;increase the proportion of lymphocyte in bone marrow;increase the number and proportion of peripheral blood lymphocytes;reduce the ratio of T lymphocytes(CD4+/CD8+)in spleen;improve the proliferation of T lymphocyte in spleen;reduce cell apoptosis in spleen tissue;increased spleen tissue proliferation related protein p-PI3K and p-AKT expression.[Conclusion]Polygonatum sibiricum Red.can improve the immune dysfunction caused by kidney essence deficiency due to sexual overstrain in middle-aged rats by affecting the proportion of T lymphocytes and their subpopulations,and its mechanism may be related to regulating the spleen tissue proliferation related protein expression of PI3K/AKT.
