UPLC-Q/TOF-MSE Analysis of the Chemical Composition of Jiangshan Polygonatum Sibiricum and Its Antioxidant and Anti-inflammatory Activities
10.16466/j.issn1005-5509.2024.07.001
- VernacularTitle:江山黄精化学成分UPLC-Q/TOF-MSE分析及其抗氧化和抗炎活性研究
- Author:
Ziye XU
1
;
Youliang WU
;
Yihuan WANG
Author Information
1. 浙江中医药大学药学院 杭州 310053
- Keywords:
UPLC-Q/TOF-MSE;
Jiangshan Polygonatum sibiricum;
antioxidant;
anti-inflammatory;
THP-1 cells;
NLRP3 inflammasome
- From:
Journal of Zhejiang Chinese Medical University
2024;48(7):761-774
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the chemical compositions,antioxidant and anti-inflammatory activities of Jiangshan Polygonatum sibiricum alcoholic extract(PSAE)in vitro.[Methods]The chemical compositions of PSAE were identified by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q/TOF-MSE),its antioxidant activity was studied,and in vitro experiments were conducted to determine its effect on lipopolysaccharide(LPS)-induced THP-1 cells.Molecular docking was further used to validate the results.[Results]PSAE contained 17 chemical compositions,including 5 flavonoids,5 saponins,3 lignans,1 phenolic acid,1 steroid and 2 other compositions.In vitro experiments showed that PSAE possessed great antioxidant activity.Besides,PSAE could increase the viability of LPS-induced THP-1 cells,significantly decrease the levels of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)in cell supernatants and the expression of NOD-like receptor thermal protein domain associated protein 3(NLRP3),apoptosis-associated speck-like protein containing CARD(ASC)and cysteinyl aspartate specific proteinase-1(Caspase-1).Molecular docking results showed that 16 of the 17 chemical compositions detected in PSAE had good docking activities with the core targets of NLRP3,ASC and Caspase-1.[Conclusion]PSAE has great antioxidant and anti-inflammatory activities,and the main active compositions may be flavonoids and saponins.
