Study on the Effect of Modified Duanggui Buxue Decoction on the Immune Function of Normal Mice
10.16466/j.issn1005-5509.2024.05.001
- VernacularTitle:加味当归补血汤对正常小鼠免疫功能的作用研究
- Author:
Xueling HU
1
;
Jie SU
;
Meiqiu YAN
Author Information
1. 浙江中医药大学 杭州 310053
- Keywords:
modified Duanggui Buxue Decoction;
normal;
immune organ;
immune function;
lymphocytes;
spleen
- From:
Journal of Zhejiang Chinese Medical University
2024;48(5):517-529
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the implication of modified Duanggui Buxue Decoction on the immune function of normal mice.[Methods]A total of 120 BALB/c mice were stochastically divided into three groups,immunization group Ⅰ,immunization group Ⅱ and immunization group Ⅲ.According to body weight,each immune group was randomly divided into 4 groups:normal control group,modified Duanggui Buxue Decoction 1.4 g·kg-1 group,modified Duanggui Buxue Decoction 1.9 g·kg-1 group,modified Duanggui Buxue Decoction 2.3 g·kg-1 group.After being gavaged for 30 d,for immune group Ⅰ:test the behaviors,spleen index,thymus index,the proliferative capacity of splenic T lymphocytes,NK cell activities,blood indices,serum levels of immunoglobulin M(IgM)and immunoglobulin G(IgG),the ratios of T and B lymphocytes in the peripheral blood and spleen.Immune group Ⅱ:number of hemolytic vacant spots and 50%haemolysis requirement for complement(HC50).Immune group Ⅲ:phagocytosis of phagocytic chicken erythrocytes in mice peritoneal macrophages and expression of CD4 and CD8 in spleen.[Results]Compared with the normal control group,all three doses of modified Duanggui Buxue Decoction significantly elevated the number of voluntary activities and grip strength,spleen index and thymus index of mice(P<0.05,P<0.01);enhanced the proliferative capacity of splenic lymphocytes and the activity of NK cells(P<0.01);increased the number of hemolytic vacant spots,HC50,the phagocytosis rate of chicken erythrocytes and phagocytosis index of the peritoneal macrophage(P<0.01);elevated whole blood lymphocyte(LYMPH),white blood cell(WBC)and red blood cell(RBC)counts,and serum IgM and IgG content(P<0.05,P<0.01);and decreased the proportions of CD3+T and CD8+T cells in blood and spleen,and decreased the amount of CD8 positive expression(P<0.05,P<0.01).Elevated peripheral blood CD19+B cell percentage,peripheral blood and spleen CD4+/CD8+ratio and spleen CD4/CD8 positive expression ratio(P<0.05,P<0.01).[Conclusion]The modified Duanggui Buxue Decoction has the function of enhancing specific immunity and non-specific immunity,which contributes to enhancing immunity.
