Mechanism of porphyridium cruentum polysaccharides extract in reducing telangiectasia
10.3760/cma.j.issn.1671-0290.2024.05.005
- VernacularTitle:紫球藻多糖提取物祛除毛细血管扩张作用机制的实验研究
- Author:
Zhongcai JIN
1
;
Jingjing LI
;
Feifei WANG
;
Li HE
Author Information
1. 云南特色植物提取实验室 皮肤健康研究院，昆明 650106
- Keywords:
Telangiectasis;
Porphyridium;
Angiogenesis;
Vasoconstriction;
Endothelin-1
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2024;30(5):435-440
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanism of Porphyridium cruentum polysaccharides extract in reducing telangiectasis.Methods:Experiments were conducted between January 10, 2023, and April 18, 2023, at the Yunnan Characteristic Plant Extraction Laboratory. In vitro experiments were conducted to investigate the effect of different concentrations of Porphyridium cruentum polysaccharides extract (Porphyridium cruentum Pro) on tube formation and migration of microvascular endothelial cells, using tube formation assay and migration assay. In an in vivo experiment, a chick embryo chorioallantoic membrane assay was conducted to assess the impact of Porphyridium cruentum Pro on vasoconstriction. Finally, a western blot analysis was conducted to confirm the impact of Porphyridium cruentum Pro on increasing the protein level of endothelin-1 in microvascular endothelial cells.Results:When the concentration of Porphyridium cruentum Pro was below 1.0%, it did not significantly affect all the stages of tube formation and the migration ability of microvascular endothelial cells ( P>0.05). However, at a concentration of 3.0%, there was a noticeable effect on the collapse of the formed tubes. In comparison to the control group, some tubes started collapsing 24 hours post-treatment, despite the lack of statistical significance in the analysis. After 48 hours of treatment, all the tubes collapsed, as evidenced by a significant decrease in the number of tubes ( t=9.02, P<0.05) and total tube length ( t=10.89, P<0.05) per field. The migration assay study revealed that at a concentration of 3%, there was observed cell detachment and atrophy, while the cell migration capacity of microvascular endothelial cells remained relatively unchanged. When treated with a 3.0% concentration of Porphyridium cruentum Pro for 30 minutes, a noteble vasoconstriction was observed in the blood vessels of the chick embryo chorioallantoic membrane. Moreover, after a 24-hour treatment with 0.3% Porphyridium cruentum Pro, there was an elevation in endothelin-1 protein expression level in microvascular endothelial cells. Conclusions:The observed function of Porphyridium cruentum Pro in reducing telangiectasis may associated to its ability to enhance the expression of endothelin-1 in vascular endothelial cells, which in turn promotes the vasoconstriction. Meanwhile, the angiogenesis is not significantly affected by Porphyridium cruentum Pro.
