Establishment and validation of reference intervals for reticulocyte parameters in children from Beijing
10.3760/cma.j.cn114452-20231115-00286
- VernacularTitle:北京地区儿童网织红细胞参数参考区间的建立及验证
- Author:
Fang JIN
1
;
Wenqi SONG
;
Yaguang PENG
;
Chaonan HE
Author Information
1. 国家儿童医学中心 首都医科大学附属北京儿童医院，临床检验中心，北京 100045
- Keywords:
Children;
Reticulocytes;
Reticulocyte parameters;
Reference interval
- From:
Chinese Journal of Laboratory Medicine
2024;47(5):554-560
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the reference intervals of reticulocyte parameters for children in the Beijing area.Methods:1 766(856 males and 910 females) healthy children aged from 1 to 18 years old in the Beijing area were studied, the infant group (≥1-3 years old) included 146 participants; the preschool group (>3-6 years old) had 449 participants; the school age group (>6-13 years old) had 646 participants and the adolescent group (>13-18 years old) had 525 participants. Seven parameters were measured in venous blood by SySmex XN-20 (A1) automatic blood cell analyzer, which included reticulocyte percentage (RET%), absolute reticulocyte count (RET#), low fluorescence reticulocyte (LFR), medium fluorescence reticulocyte (MFR), high fluorescence reticulocyte (HFR), immature reticulocyte fraction (IRF) and reticulocyte hemoglobin equivalent (RET-He). After the test results were collected, the reference intervals were established according to the percentile ( P2.5, P97.5). As the reference intervals were established, venous blood samples from 109 healthy children in Beijing were collected to verify the reference intervals according to WS/T 402-2012 "Define and determine the reference intervals in clinical laboratory". Results:The reference interval of 7 Reticulocyte parameters for children aged 1-18 years without age and sex grouping,reference interval (RET%): 0.74%-2.06%; absolute reticulocyte count (RET#): (34.5-101.5)×10 9/L; low fluorescence reticulocyte (LFR): 86.6%-96.5%; medium fluorescence reticulocyte (MFR): 3.2%-11.5%; high fluorescence reticulocyte (HFR): 0%-2.1%; immature reticulocyte fraction (IRF): 3.5%-13.4%; reticulocyte hemoglobin equivalent (RET-He): 28.5-34.2 pg. Over 90% test results of samples from the 109 children ranged within the reference ranges. Conclusion:This study established convincible reference intervals of seven reticulocyte parameters for healthy children aged from 1 to 18 in the Beijing area was established, and reference interval verification passed.
