Protective Effect of Allium tuberosum Extract on Vascular Inflammation in Tumor Necrosis Factor-α-induced Human Vascular Endothelial Cells.
10.15430/JCP.2017.22.4.228
- Author:
Haeng Jeon HUR
1
;
Ae Sin LEE
Author Information
1. Korea Food Research Institute, Wanju, Korea. aslee@kfri.re.kr
- Publication Type:Original Article
- Keywords:
Allium tuberosum extract;
Vascular cell adhesion molecule-1;
Intercellular adhesion molecule-1;
Inflammation;
NF-kappa B
- MeSH:
Allium*;
Ascorbic Acid;
Atherosclerosis;
Blood Circulation;
Blotting, Western;
Calcium;
Carotenoids;
Chive*;
Endothelial Cells*;
Functional Food;
Gene Expression;
Human Umbilical Vein Endothelial Cells;
Humans*;
Inflammation*;
Intercellular Adhesion Molecule-1;
Iron;
Metabolism;
Monocytes;
Necrosis*;
NF-kappa B;
Phosphorus;
Phosphorylation;
Polymerase Chain Reaction;
Reverse Transcription;
RNA, Messenger;
Vascular Cell Adhesion Molecule-1;
Vascular Diseases;
Vitamin A
- From:Journal of Cancer Prevention
2017;22(4):228-233
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Endothelial adhesion molecule expression induced by pro-inflammatory cytokine plays an important role in vascular endothelial cell injury, leading to vascular disease. Allium tuberosum (AT), which is used as a functional food, has a thrombolytic effect. It contains vitamin A, vitamin C, carbohydrate, calcium, iron, and phosphorus. There are many carotenes that turn into vitamin A in the body. Also, it helps blood circulation and stimulates metabolism. The purpose of the this study was to estimate the anti-inflammatory effects of the AT extract. METHODS: Human vascular endothelial cells were pre-treated with 100 μg/mL AT extract for 30 minutes and subsequently co-treated with TNF-α (10 ng/mL) and AT extract (100 μg/mL) for 1, 4, and 6 hours. After treatment, the cells were lysed and used for quantitative reverse transcription PCR, Western blot analysis, and monocyte adhesion assay. RESULTS: We examined the effect of the AT extract on inflammatory gene expression in TNF-α-induced human umbilical vein endothelial cells (HUVECs). The extract reduced the expression levels of mRNA and protein of intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 in TNF-α-stimulated HUVECs. It also inhibited the TNF-α-induced phosphorylation of the NF-κB p65 subunit and degradation of IκBα. Furthermore, the AT extract prevented the increased adhesion capacity of monocyte to TNF-α-stimulated vascular endothelial cells by reducing ICAM-1 and VCAM-1 expression. CONCLUSIONS: The AT extract has preventive and anti-inflammatory effect against vascular disease and has potential for supporting prevention against the early process of atherosclerosis.
