TP53 R72P Polymorphism and Susceptibility to Human Papillomavirus Infection Among Women With Human Immunodeficiency Virus in Morocco: A Case-control Study.
10.15430/JCP.2017.22.4.248
- Author:
Ahd Oulad LAHSEN
1
;
Hanâ BABA
;
Rajaa BENSGHIR
;
Naouar FAYSSEL
;
Mustapha SODQI
;
Latifa MARIH
;
Sellama NADIFI
;
Lahcen WAKRIM
;
Kamal Marhoum EL FILALI
;
Sayeh EZZIKOURI
Author Information
1. Service des Maladies Infectieuses, CHU Ibn Rochd, Casablanca, Morocco.
- Publication Type:Original Article
- Keywords:
P53 codon 72;
Human papillomavirus;
Polymorphism;
Susceptibility;
Human immunodeficiency virus
- MeSH:
Alleles;
Case-Control Studies*;
DNA;
Female;
Gene Frequency;
Genes, Tumor Suppressor;
Genotype;
HIV*;
Humans*;
Life Style;
Logistic Models;
Morocco*;
Papillomavirus Infections*;
Uterine Cervical Neoplasms
- From:Journal of Cancer Prevention
2017;22(4):248-253
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Human papillomavirus (HPV) is the most common sexually transmitted agent worldwide. HPV is the main causative agent for cervical cancer. The HPV oncoprotein E6 binds to the tumor suppressor gene product p53, promoting its degradation; the Arg allele of TP53 R72P polymorphism binds more ardently with HPV E6 than the Pro variant. Here, we investigated whether TP53 R72P gene variant, rs104252, was associated with susceptibility to HPV infection in women with human immunodeficiency virus (HIV). METHODS: We analyzed 200 HPV-positive and 68 uninfected women with HIV. Genomic DNA was isolated from cervical swab. The TP53 R72P polymorphism was genotyped by PCR-RFLP. Unconditional logistic regression was used to assess the association between polymorphism and the clinical, lifestyle, and behavioral data. RESULTS: The genotype and allele frequencies of rs104252 variant did not differ between women without or with HPV infection (P > 0.05). Moreover, the p53 polymorphism was not associated with cervical cytology. In contrast, when we analyzed according to behavior factors, the P72P genotype was more frequent among HPV-positive smoker women. However, no significant relationship was found between alcohol, contraceptive use, and number of partners with TP53 R72P genotype distributions among HPV-positive cases (P > 0.05). CONCLUSIONS: The R72 variant of p53 R72P is not associated with HPV infection and progression of lesions. There was no association between this variant and behavior factors in HPV-positive cases. The P72P genotype may be more frequent among HPV-positive smoker women.
