Analysis of pulmonary microbial characteristics in patients with early-stage lung adenocarcinoma
10.3760/cma.j.cn112434-20240418-00107
- VernacularTitle:早期肺腺癌患者肺部微生物特征分析
- Author:
Fei QI
1
;
Hongxiang FENG
;
Jin ZHANG
;
Weijie ZHU
;
Yang HAO
;
Fei XIAO
;
Yuhui SHI
;
Chaoyang LIANG
;
Deruo LIU
;
Zhenrong ZHANG
Author Information
1. 中日友好医院（中日友好临床医学研究所） 北京协和医学院 中国医学科学院，北京 100029
- Keywords:
Lung adenocarcinoma;
Microbiota;
Bronchoalveolar lavage fluid
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2024;40(11):647-653
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore whether lung microorganisms influence the occurrence and development of early lung adenocarcinoma by analyzing the presence and changes of local microorganisms in early lung adenocarcinoma.Methods:Tumor tissues and alveolar lavage fluid were collected from 32 patients diagnosed with lung adenocarcinoma at the China-Japan Friendship Hospital. Nextseq550DX was used for DNA sequencing. R language was used to calculate the number of microorganisms in the tumor tissue and alveolar lavage fluid samples of different stages and grades. species and diversity, and use statistical methods such as TSEA and LEfSe analysis to compare the diversity and differences of microorganisms in different groups. Results:Both Actinobacteria and Proteobacteria accounted for a higher proportion in both the tumor tissue and alveolar lavage fluid samples of patients with lung adenocarcinoma. The number of microorganisms detected in the tumor tissue was greater than that in the lavage fluid, and the alpha diversity was higher, but the difference Not significant( P<0. 05). According to the analysis of differences in lung microorganisms in patients with different pathological grades: In the two samples, invasive adenocarcinoma has more microorganisms than carcinoma in situ + microinvasive carcinoma, and the alpha diversity of the lavage fluid is significantly higher, and the comparison between the groups is statistically significant( P=0. 011); the difference in β diversity was not statistically significant( P>0. 05). Group analysis based on different pathological types: the invasive adenocarcinoma group samples showed a decrease in Proteobacteria, the carcinoma in situ + minimally invasive cancer group was mainly composed of Proteobacteria, and most of the microorganisms related to lung cancer differences analyzed in each group belonged to Proteobacteria and Actinobacteria. Phylum. Conclusion:Most of the microbes in tumor tissues and lavage fluid of lung adenocarcinoma belong to the Actinobacteria and Proteobacteria. Most of the differentially associated microbes of lung cancer under different classifications also belong to Proteobacteria and Actinobacteria, which are the main characteristics of lung microbial composition in patients with early-stage lung adenocarcinoma.
