Epidemiological analysis of unique recombinant strains in newly diagnosed HIV-1 patients in Hangzhou
10.3760/cma.j.cn112309-20240306-00072
- VernacularTitle:杭州市新确诊HIV-1感染者独特重组毒株流行情况分析
- Author:
Ling YE
1
;
Ke XU
;
Wenjie LUO
;
Xingliang ZHANG
;
Sisheng WU
;
Min ZHU
;
Jia′nyu YOU
Author Information
1. 杭州市疾病预防控制中心（杭州市卫生监督所）艾滋病性病防制所，杭州 310002
- Keywords:
Human immunodeficiency virus;
Unique recombinant form;
Surveillance drug resistance mutation;
Molecular network
- From:
Chinese Journal of Microbiology and Immunology
2024;44(11):958-964
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To understand the molecular epidemiological characteristics of unique recombinant forms (URFs) in newly reported HIV-1 patients in Hangzhou, and provide theoretical support for prevention and control of AIDS.Methods:The blood samples of newly-diagnosed HIV-1 infected cases who received no antiviral therapy from 2019 to 2023 were collected, pol gene was amplified by RT-PCR and nested PCR, followed by sequencing. The URFs were screened using phylogenetic tree, followed by recombinant analysis. Genetic distances between URFs sequences were calculated and molecular transmission networks were constructed. The calibrated population resistance program (CPR) was used to analyze transmissible drug-resistant mutations. Results:A total of 222(5.0%, 222/4 471) URFs pol gene sequence were obtained, and the recombination types were CRF01_AE/CRF07_BC (60.4%, 134/222), CRF01_AE/C (11.7%, 26/222), CRF01_AE/B (9.5%, 21/222), CRF01_AE/B/C (8.1%, 18/222), B/C (7.6%, 17/222) and CRF55_01B/CRF07_BC (2.7%, 6/222), respectively. 78.8% (175/222) were infected by men who have sex with man(MSM), whoes mean age was 31.3±10.5. The proportion of URFs increased from 4.0% (34/843) to 7.4% (60/807) from 2019 to 2023. Under the optimal gene distance threshold of 1.5%, the molecular network access rate was 49.5% (110/222), included 23 clusters. We found a large active transmission cluster with 39 cases mixed homosexual and heterosexual, the recombination types was CRF01_AE/CRF07_BC, and the average gene distance was 0.005. Prevalence of URFs transmissible resistance was 3.2% (7/222). Conclusions:URFs are mainly produced and transmitted in young MSM, which shows an increasing trend year by year. There is a large active transmission cluster required major attention and effective intervention to prevent further expansion. At the same time, the occurrence and transmission of URFs should be continuously monitored to understand the clusters and drug resistance dynamics.
