Analysis of the pathogen spectrum of hand, foot and mouth disease and the genetic characteristics of coxsackievirus A6 in Qingdao from 2020 to 2022
10.3760/cma.j.cn112309-20240219-00052
- VernacularTitle:2020—2022年青岛地区手足口病病原谱及柯萨奇病毒A6型青岛株VP1区基因特征分析
- Author:
Jinling GONG
1
;
Siying XIAO
;
Rongxiang LIANG
;
Shunxin ZHU
;
Rui SUN
Author Information
1. 山东省青岛市疾病预防控制中心病毒性疾病检验所，青岛 266000
- Keywords:
Hand, foot and mouth disease;
Pathogenic spectrum;
Epidemiological characteristics;
Coxsackievirus A6;
Phylogenetic analysis
- From:
Chinese Journal of Microbiology and Immunology
2024;44(8):713-717
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the etiological characteristics of hand, foot and mouth disease (HFMD) and the VP1 gene evolution of coxsackievirus A6 (CVA6) in Qingdao from 2020 to 2022.Methods:Total enterovirus (EV), enterovirus 71 (EV71), coxsackievirus A16 (CVA16), CVA6 and coxsackievirus A10 (CVA10) were detected by fluorescent RT-PCR in throat swab samples from HFMD patients in Qingdao from 2020 to 2022. The full-length sequence amplification of VP1 gene and gene sequence determination of CVA6 isolates were performed, and the phylogenetic and molecular characteristics were analyzed by MEGA-X software.Results:The overall positive rate of enteroviruses in 1 645 samples collected during 2020-2021 was 80.73%(1 328/1 645). The proportions of EV71, CVA16, CVA6, CVA10 and other enteroviruses were 0.08%(1/1 328), 19.65%(261/1 328), 68.15%(905/1 328), 0.98%(13/1 328) and 11.14%(148/1 328), respectively. Children under 5 years old accounted for 75.68% (1 245/1 645) of all cases and 100% (2/2) of severe cases. A phylogenetic tree was constructed based on the VP1 region sequences of 45 CVA6 strains showing all strains belonging to the D3a subtype. There were 10 non-synonymous mutations of amino acids in the VP1 protein by gene evolution analysis.Conclusions:CVA6 D3a subtype is the main epidemic strain causing HFMD in Qingdao from 2020 to 2022, especially in children under 5 years old.
