Identification and phylogenetic analysis of one clinical isolate of Ochrobactrum teleogrylli from human blood
10.3760/cma.j.cn112309-20231208-00171
- VernacularTitle:1株血液分离的油葫芦苍白杆菌的鉴定及系统发育分析
- Author:
Jianlong LIU
1
;
Dexing HAN
;
Shunguang LI
;
Ya LIU
;
Chunrong SONG
;
Xiaowei CHEN
;
Min FU
;
Qiong HU
;
Pinghua QU
Author Information
1. 湖南省儿童医院检验中心，长沙 410007
- Keywords:
Ochrobactrum;
16S rRNA;
Whole-genome sequencing;
Phylogenetic tree
- From:
Chinese Journal of Microbiology and Immunology
2024;44(8):680-688
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the biological characteristics, phylogeny and the taxonomic status of strain 7712 (=CGMCC 1.17031=NBRC 113783=KCTC 15766) isolated from a clinical blood sample.Methods:Strain 7712 was identified by the cultural properties, cellular and colonial morphology, physiological and biochemical reactions, matrix-assisted laser desorption ionization time-of-flight mass spectrometry system, and genome correlation index analysis. The genomic phylogenetic tree was construct to analyze the taxonomic position. The virulence factors and resistance genes of strain 7712 and related strains were then compared by the online virulence factor database and online comprehensive antibiotic research database respectively.Results:Strain 7712 was urease negative, gram-negative nonfermenters, which was identified as Ochrobactrum anthropi by VITEK GN card. The 16S rRNA gene analysis showed that the strain was closely related to the members of genera Ochrobactrum and Brucella. The phylogenetic tree showed that strain 7712 was clustered together with Ochrobactrum teleogrylli LCB8 T and Ochrobactrum haematophilum CCUG 38531 T, along with genus Brucella and other Ochrobactrum species. The genome relatedness indexes analysis showed that the average nucleotide identity between strain 7712 and Ochrobactrum teleogrylli LCB8 T was 98.16%, which was higher than the threshold for prokaryotic species. Genetic prediction showed that strain 7712 carried several virulence-related genes and resistance-related genes, of which the existence of OCH gene might be responsible to the resistance to cephalosporin. Conclusions:A case of human infection caused by Ochrobactrum teleogrylli is identified, which would help promote the understanding of biodiversity of genus Ochrobactrum.
