Epidemiological and molecular characteristics of human metapneumovirus in children in Tianjin
10.3760/cma.j.cn112309-20240318-00100
- VernacularTitle:天津市儿童中人偏肺病毒的流行病学及分子特征分析
- Author:
Rui LI
1
;
Liru GUO
;
Mei KONG
;
Ming ZOU
;
Xiaochang LIU
;
Tao WANG
;
Mengxue GAO
;
Xiaoyan LI
Author Information
1. 天津医科大学公共卫生学院，天津 300070
- Keywords:
Human metapneumovirus;
Epidemiological characteristics;
Phylogenetic analysis;
Respiratory infection
- From:
Chinese Journal of Microbiology and Immunology
2024;44(7):588-595
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the epidemiological and genetic characteristics of human metapneumovirus (hMPV) in Tianjin during two influenza epidemic seasons from October 2020 to March 2021 and from October 2021 to March 2022, and enrich the whole genome database of hMPV in China.Methods:A total of 1 040 pharyngeal swab samples collected from patients with influenza-like illness (ILI) were analyzed using microfluidic chip fluorescence quantitative PCR. RT-PCR was used to amplify the whole genome in hMPV-positive samples, and the second-generation sequencing was performed for complete genome sequencing. Bioinformatics software including CLC, DNAStar, and MEGA was used for sequence assembly, nucleotide and amino acid homology analysis, and phylogenetic tree mapping.Results:Among the 1 040 samples, 25 were positive for hMPV with a positive rate of 2.40%. The highest positive rate was observed in the age group of 3 to 5 years, reaching 3.71% (16/431). During the influenza epidemic seasons, the detection rate of hMPV peaked in December, reaching 6.67% (12/180). Twelve strains were successfully sequenced, and there were seven of type B2, four of type A2b, and one of type B1. More variations were detected in the G gene, with 111nt-dup sequence repeats observed in the G gene of three A2b strains.Conclusions:The prevalence of hMPV peaks in December during the influenza epidemic seasons in Tianjin, with Type B2 being the predominant type. Except for the G gene with more mutations, other genes remain stable.
